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Impact of veA on the development, aggressiveness, dissemination and secondary metabolism of Penicillium expansum

El Hajj Assaf, Christelle and Snini, Selma and Tadrist, Souria and Bailly, Sylviane and Naylies, Claire and Oswald, Isabelle P. and Pascal-Lorber, Sophie and Puel, Olivier Impact of veA on the development, aggressiveness, dissemination and secondary metabolism of Penicillium expansum. (2018) Molecular Plant Pathology, 19 (8). 1971-1983. ISSN 1464-6722

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Official URL: https://doi.org/10.1111/mpp.12673

Abstract

Penicillium expansum, the causal agent of blue mould disease, produces the mycotoxins patulin and citrinin amongst other secondary metabolites. Secondary metabolism is associated with fungal development, which responds to numerous biotic and abiotic external triggers. The global transcription factor VeA plays a key role in the coordination of secondary metabolism and differentiation processes in many fungal species. The specific role of VeA in P. expansum remains unknown. A null mutant PeΔveA strain and a complemented PeΔveA:veA strain were generated in P. expansum and their pathogenicity on apples was studied. Like the wild‐type and the complemented strains, the null mutant PeΔveA strain was still able to sporulate and to colonize apples, but at a lower rate. However, it could not form coremia either in vitro or in vivo, thus limiting its dissemination from natural substrates. The impact of veA on the expression of genes encoding proteins involved in the production of patulin, citrinin and other secondary metabolites was evaluated. The disruption of veA drastically reduced the production of patulin and citrinin on synthetic media, associated with a marked down‐regulation of all genes involved in the biosynthesis of the two mycotoxins. Moreover, the null mutant PeΔveA strain was unable to produce patulin on apples. The analysis of gene expression revealed a global impact on secondary metabolism, as 15 of 35 backbone genes showed differential regulation on two different media. These findings support the hypothesis that VeA contributes to the pathogenicity of P. expansum and modulates its secondary metabolism.

Item Type:Article
HAL Id:hal-02061925
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Other partners > Flanders Research Institute for agriculture, fisheries and food - ILVO (BELGIUM)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
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Deposited On:08 Mar 2019 13:08

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