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Nitrate and nitrite reduction at high pH in a cementitious environment by a microbial microcosm

Durban, Nadège and Rafrafi, Yan and Rizoulis, Athanasios and Albrecht, Achim and Robinet, Jean-Charles and Lloyd, Jonathan R. and Bertron, Alexandra and Erable, Benjamin Nitrate and nitrite reduction at high pH in a cementitious environment by a microbial microcosm. (2018) International Biodeterioration & Biodegradation, 134. 93-102. ISSN 0964-8305

(Document in English)

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Official URL: https://doi.org/10.1016/j.ibiod.2018.08.009


The possible release of oxyanions, such as nitrate, from radioactive waste repositories may influence redox-conditions of the near field environment and thus promote mobility of some redox sensitive radionuclides. The fate of dissolved oxyanions will be significantly conditioned by microbial activities, if present in the aqueous interstitial phase of a waste cell. This study investigates microbial nitrate reduction in a cementitious environment. A consortium of microorganisms was used, an inoculum prepared with sediments collected from a former lime works site, characterized by a pH of pore water of 11–12. The biomass was acclimated to cement leachate supplemented with nitrate, acetate and yeast extract. According to experiments performed in closed and in dynamic systems, the microbial consortium was adapted to reduce nitrate and nitrite in a cementitious, anaerobic environment (pH 11, with and without hardened cement paste and leachate). Although, nitrite accumulation was observed in close system and temporally in dynamic system. The rate of nitrate reduction was between 0.12 and 0.75 mM/h with incoming nitrate concentrations between 6 and 48 mM, respectively. The microorganism diversity and the biofilm present on the hardened cement paste helped maintain microbial activity in all of the conditions simulating cementitious environments.

Item Type:Article
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Agence Nationale pour la Gestion des Déchets Radioactifs - ANDRA (FRANCE)
French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Institut National des Sciences Appliquées de Toulouse - INSA (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Other partners > University of Manchester (UNITED KINGDOM)
Laboratory name:
Deposited On:04 Dec 2018 13:55

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