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Antifungal properties of an actinomycin D-producing strain,Streptomycessp. IA1, isolated from a Saharan soil

Toumatia, Omrane and Yekkour, Amine and Goudjal, Yacine and Riba, Amar and Coppel, Yannick and Mathieu, Florence and Sabaou, Nasserdine and Zitouni, Abdelghani Antifungal properties of an actinomycin D-producing strain,Streptomycessp. IA1, isolated from a Saharan soil. (2014) Journal of Basic Microbiology, 55 (2). 221-228. ISSN 0233-111X

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Official URL: https://doi.org/10.1002/jobm.201400202


An actinomycete strain named IA1, which produced an antimicrobial compound, was isolated from a Saharan soil in In Amenas, Algeria. The study of the 16S rDNA sequence of this strain permitted to relate it to Streptomyces mutabilis NBRC 12800T (99.93% of similarity). Strain IA1 exhibited strong activity against a wide range of plant pathogenic fungi. One bioactive compound produced in large amounts (46.7 mg L−1 day−1), named YA, was isolated and purified by TLC and reverse phase HPLC. The structure elucidation of the pure substance, using combined data from UV visible, NMR spectra, and mass spectrometry, permitted to identify it as actinomycin D, and was thus found for the first time in S. mutabilis related species. The biocontrol abilities of the strain IA1 and compound YA were evaluated through two diseases, i.e., chocolate spot of field bean and Fusarium wilt of flax. The occurrence of the two fungal diseases was effectively reduced. The reduction of chocolate spot disease symptoms reached 80 and 91.7% with IA1 and YA seedlings pretreatments, respectively. Soil pretreatment with IA1 or YA also allowed to reduce Fusarium wilt disease impact by almost 60%.

Item Type:Article
HAL Id:hal-01938006
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Other partners > Ecole normale supérieure de Kouba (ALGERIA)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Other partners > Université M'sila (ALGERIA)
Laboratory name:
Deposited On:01 Oct 2018 13:53

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