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The centrifugal partition reactor, a novel intensified continuous reactor for liquid–liquid enzymatic reactions

Nioi, Claudia and Riboul, David and Destrac, Philippe and Marty, Alain and Marchal, Luc and Condoret, Jean-Stéphane The centrifugal partition reactor, a novel intensified continuous reactor for liquid–liquid enzymatic reactions. (2015) Biochemical Engineering Journal, 103. 227-233. ISSN 1369-703X

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Official URL: https://doi.org/10.1016/j.bej.2015.07.018


Implementation of continuous processes for production of fine chemicals and pharmaceuticals is an efficient way for process intensification. This study aims at demonstrating the potential of a Centrifugal Partition Chromatography (CPC) apparatus as a novel type of intensified reactor (termed Centrifugal Partition Reactor, CPR) for biphasic (water-organic solvent) enzymatic reactions. The reaction of esterification of oleic acid with n-butanol catalyzed by the Rhizomucor miehei lipase was tested as the model reaction. The influence of rotation speed, flow rate, enzyme and substrate concentrations on esterification reaction were studied. The CPR proved to be efficient to generate sufficient interfacial area (weakly dependent of the flow rate) and sufficient residence time (30 min) to achieve good conversion (85%). Also, increasing rotation speed of the CPR surprisingly decreased performances, probably due to very specific inner hydrodynamics. For a given configuration, the productivity of the CPR (40.5 g h−1 L−1) was found to be more favorable than the conventional batch process (21.6 g h−1 L−1). Steady state operation of the reactor at 22 °C, (i.e., constant conversion at the output, see Fig. 8), was reached after about 2 residence times and lasted for 24 h. After 24 h, the output conversion slowly decreased due to the low intrinsic stability of the enzyme at room temperature. The promising results obtained in this study are a good incentive to promote the CPR as a competitive innovative technology for operating continuous two phase enzymatic reactions.

Item Type:Article
HAL Id:hal-01913310
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Other partners > Ecole des Mines de Nantes (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Université de Toulouse > Institut National des Sciences Appliquées de Toulouse - INSA (FRANCE)
Other partners > Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique - ONIRIS (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Other partners > Université de Nantes (FRANCE)
Other partners > IMT Atlantique Bretagne-Pays de la Loire - IMT Atlantique (FRANCE)
Laboratory name:
French National Research Agency (ANR)
Deposited On:05 Nov 2018 08:05

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