Jovani-Abril, Raquel and Gibilaro, Mathieu and Janssen, Arne and Eloirdi, Rachel and Somers, Joseph and Spino, José L. and Malmbeck, Rikard
Synthesis of nc-UO 2 by controlled precipitation in aqueous phase.
(2016)
Journal of Nuclear Materials, 477. 298-304. ISSN 0022-3115
|
(Document in English)
PDF (Author's version) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader 1MB |
Official URL: https://doi.org/10.1016/j.jnucmat.2016.04.050
Abstract
A new strain of actinobacteria, designated ACD1, was isolated from a Saharan soil sample in the Hoggar region (Algeria). Morphological study led to this strain being classified as a member of the Actinomadura genus. Phylogenetic analysis based on the 16S rRNA gene showed that the strain is closely related to Actinomadura sediminis DSM 45500T (98.5% sequence similarity). Furthermore, strain ACD1 presented a strong activity against mycotoxigenic and phytopathogenic fungi, including Aspergillus and Fusarium strains, and other pathogenic microorganisms. The kinetics of antimicrobial activity were investigated on ISP-2, Bennett and TSB media. Four solvents (n-hexane, dichloromethane, ethyl acetate and n-butanol) were used for the extraction of the produced antibiotic. The highest antimicrobial activity was obtained using the butanolic extract from the ISP-2 medium after seven days of fermentation culture. The active antibiotic was purified by reverse-phase HPLC using a C18 column. The UV-visible and mass spectra were determined. The minimum inhibitory concentrations (MIC) of this antibiotic were determined against pathogenic microorganisms.
Item Type: | Article |
---|---|
HAL Id: | hal-02134867 |
Audience (journal): | International peer-reviewed journal |
Uncontrolled Keywords: | |
Institution: | French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE) Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE) Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE) Other partners > European Commission, Joint Reasearch Centre - JRC (GERMANY) |
Laboratory name: | |
Statistics: | download |
Deposited On: | 25 Oct 2018 08:48 |
Repository Staff Only: item control page