Lahoum, Abdelhadi and Aouiche, Adel and Bouras, Noureddine and Verheecke, Carol and Klenk, Hans-Peter and Sabaou, Nasserdine and Mathieu, Florence
Antifungal activity of a Saharan strain of Actinomadura sp. ACD1 against toxigenic fungi and other pathogenic microorganisms.
(2016)
Journal de Mycologie Médicale / Journal of Medical Mycology, 26 (3). 193-200. ISSN 1156-5233
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(Document in English)
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Official URL: https://doi.org/10.1016/j.mycmed.2016.02.017
Abstract
A new strain of actinobacteria, designated ACD1, was isolated from a Saharan soil sample in the Hoggar region (Algeria). Morphological study led to this strain being classified as a member of the Actinomadura genus. Phylogenetic analysis based on the 16S rRNA gene showed that the strain is closely related to Actinomadura sediminis DSM 45500T (98.5% sequence similarity). Furthermore, strain ACD1 presented a strong activity against mycotoxigenic and phytopathogenic fungi, including Aspergillus and Fusarium strains, and other pathogenic microorganisms. The kinetics of antimicrobial activity were investigated on ISP-2, Bennett and TSB media. Four solvents (n-hexane, dichloromethane, ethyl acetate and n-butanol) were used for the extraction of the produced antibiotic. The highest antimicrobial activity was obtained using the butanolic extract from the ISP-2 medium after seven days of fermentation culture. The active antibiotic was purified by reverse-phase HPLC using a C18 column. The UV-visible and mass spectra were determined. The minimum inhibitory concentrations (MIC) of this antibiotic were determined against pathogenic microorganisms.
Item Type: | Article |
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HAL Id: | hal-02134854 |
Audience (journal): | International peer-reviewed journal |
Uncontrolled Keywords: | |
Institution: | French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE) Other partners > Ecole normale supérieure de Kouba (ALGERIA) Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE) Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE) Other partners > Université de Ghardaïa (ALGERIA) Other partners > Newcastle University (UNITED KINGDOM) |
Laboratory name: | |
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Deposited On: | 17 Oct 2018 08:01 |
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