Driche, El Hadj and Sabaou, Nasserdine and Bijani, Christian and Zitouni, Abdelghani and Pont, Frédéric and Mathieu, Florence
and Badji, Boubekeur
Streptomyces sp. AT37 isolated from a Saharan soil produces a furanone derivative active against multidrug-resistant Staphylococcus aureus.
(2017)
World Journal of Microbiology and Biotechnology, 33 (6). 1-13. ISSN 0959-3993
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(Document in English)
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Official URL: https://doi.org/10.1007/s11274-017-2265-y
Abstract
A novel actinobacterium strain, named AT37, showed a strong activity against some multidrug-resistant Staphylococcus aureus, including methicillin-resistant S. aureus MRSA ATCC 43300, other clinical isolates of MRSA and vancomycin resistant S. aureus VRSA S1. The strain AT37 was isolated from a Saharan soil by a dilution agar plating method using chitin-vitamin agar medium supplemented with rifampicin. The morphological and chemical studies indicated that this strain belonged to the genus Streptomyces. Its 16S rRNA gene sequence was determined and a database search indicated that it was closely associated with the type strain of Streptomyces novaecaesareae NBRC 13368T with 99.6% of similarity. However, the comparison of the morphological and the physiological characteristics of the strain with those of the nearest species showed significant differences. The strain AT37 secreted the antibiotic optimally during mid-stationary phase of growth. One active compound (AT37-1) was extracted from the culture broth with dichloromethane, separated on silica gel plates and purified by HPLC. Based on spectroscopic analysis of UV-Visible, infrared, and 1H and 13C NMR spectra and spectrometric analysis, the chemical structure of the compound AT37-1 was identified as 5-[(5E,7E,11E)-2,10-dihydroxy-9,11-dimethyl-5,7,11-tridecatrien-1-yl]-2-hydroxy-2-(1-hydroxyethyl)-4-methyl-3(2H)-furanone. Minimum inhibitory concentrations and minimum biofilm inhibitory concentration (MBIC50) of this compound showed significant activity against multidrug-resistant S. aureus with 15-30 and 10-15 μg/mL, respectively.
Item Type: | Article |
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HAL Id: | hal-01948255 |
Audience (journal): | International peer-reviewed journal |
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Institution: | French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE) Other partners > Ecole normale supérieure de Kouba (ALGERIA) Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE) French research institutions > Institut National de la Santé et de la Recherche Médicale - INSERM (FRANCE) Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE) Other partners > Université Hassiba Benbouali de Chlef (ALGERIA) |
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Deposited On: | 12 Oct 2018 14:18 |
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