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Nocardiopsis algeriensis sp. nov., an alkalitolerant actinomycete isolated from Saharan soil

Bouras, Noureddine and Meklat, Atika and Zitouni, Abdelghani and Mathieu, Florence and Schumann, Peter and Spröer, Cathrin and Sabaou, Nasserdine and Klenk, Hans-Peter Nocardiopsis algeriensis sp. nov., an alkalitolerant actinomycete isolated from Saharan soil. (2015) Antonie van Leeuwenhoek, 107 (2). 313-320. ISSN 0003-6072

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Official URL: http://doi.org/10.1007/s10482-014-0329-7


An alkalitolerant actinomycete strain, designated B32T, was isolated from a Saharan soil sample collected from Adrar province (South of Algeria), and then investigated using a polyphasic taxonomic approach. The strain was observed to produce short chains of spores on the dichotomous branched aerial mycelium and formed a fragmented substrate mycelium. The optimum NaCl concentration for growth was found to be 0–5 % (w/v) and the optimum growth temperature and pH were found to be 25–35 °C and 7.0–10.0 °C, respectively. The diagnostic diamino acid in the cell-wall peptidoglycan was identified as meso-diaminopimelic acid. The predominant menaquinones of strain B32T were identified as MK-10 (H4) and MK-11 (H4). The major fatty acids were found to be iso-C16:0 and anteiso-C15:0. The diagnostic phospholipids detected were phosphatidylcholine, phosphatidylmethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The chemotaxonomic properties of strain B32T are consistent with those shared by members of the genus Nocardiopsis. 16S rRNA gene sequence analysis indicated that strain B32T is most closely related to Nocardiopsis alba DSM 43377T (98.7 %), Nocardiopsis lucentensis DSM 44048T (98.6 %), Nocardiopsis aegyptia DSM 44442T (98.6 %), Nocardiopsis sinuspersici HM6T (98.6 %) and Nocardiopsis arvandica HM7T (98.5 %). However, the DNA–DNA relatedness values between strain B32T and the closely related type strains were 17.9, 14.6, 31.1, 27.1 and 14.1 %, respectively. Based on the combined genotypic and phenotypic evidence, it is proposed that strain B32T should be classified as representative of a novel species, for which the name Nocardiopsis algeriensis sp. nov. is proposed. The type strain is B32T (=DSM 45462T = CECT 8712T).

Item Type:Article
HAL Id:hal-01894564
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Other partners > Leibniz-Institut Deutsche Sammlung von Mikroorganismen und Zelllkulturen GmBH - DSMZ (GERMANY)
Other partners > Ecole normale supérieure de Kouba (ALGERIA)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Other partners > Université de Saâd Dahlab Blida - USDB (ALGERIA)
Other partners > Université de Ghardaïa (ALGERIA)
Laboratory name:
Deposited On:12 Oct 2018 13:30

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