OATAO - Open Archive Toulouse Archive Ouverte Open Access Week

Identification of new biophysical markers for pathological ventricular remodelling in tachycardia-induced dilated cardiomyopathy

Benitez-Amaro, Aleyda and Samouillan, Valérie and Jorge, Esther and Dandurand, Jany and Nasarre, Laura and de Gonzalo-Calvo, David and Bornachea, Olga and Amoros-Figueras, Gerard and Lacabanne, Colette and Vilades, David and Leta, Ruben and Carreras, Francesc and Gallardo, Alberto and Lerma, Enrique and Cinca, Juan and Guerra, Jose M. and Llorente-Cortés, Vicenta Identification of new biophysical markers for pathological ventricular remodelling in tachycardia-induced dilated cardiomyopathy. (2018) Journal of Cellular and Molecular Medicine, 22 (9). 4197-4208. ISSN 1582-1838

(Document in English)

PDF (Publisher's version) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader

Official URL: https://doi.org/10.1111/jcmm.13699


Our aim was to identify biophysical biomarkers of ventricular remodelling in tachycardia‐induced dilated cardiomyopathy (DCM). Our study includes healthy controls (N = 7) and DCM pigs (N = 10). Molecular analysis showed global myocardial metabolic abnormalities, some of them related to myocardial hibernation in failing hearts, supporting the translationality of our model to study cardiac remodelling in dilated cardiomyopathy. Histological analysis showed unorganized and agglomerated collagen accumulation in the dilated ventricles and a higher percentage of fibrosis in the right (RV) than in the left (LV) ventricle (P = .016). The Fourier Transform Infrared Spectroscopy (FTIR) 1st and 2nd indicators, which are markers of the myofiber/collagen ratio, were reduced in dilated hearts, with the 1st indicator reduced by 45% and 53% in the RV and LV, respectively, and the 2nd indicator reduced by 25% in the RV. The 3rd FTIR indicator, a marker of the carbohydrate/lipid ratio, was up‐regulated in the right and left dilated ventricles but to a greater extent in the RV (2.60‐fold vs 1.61‐fold, P = .049). Differential scanning calorimetry (DSC) showed a depression of the freezable water melting point in DCM ventricles – indicating structural changes in the tissue architecture – and lower protein stability. Our results suggest that the 1st, 2nd and 3rd FTIR indicators are useful markers of cardiac remodelling. Moreover, the 2nd and 3rd FITR indicators, which are altered to a greater extent in the right ventricle, are associated with greater fibrosis.

Item Type:Article
Additional Information:This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Thanks to Wiley editor. The original PDF of the article can be found at : https://onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.13699
HAL Id:hal-03218495
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Laboratory name:
Deposited On:20 Jun 2018 13:52

Repository Staff Only: item control page