Huang, Baowen and Routaboul, Jean-Marc
and Liu, Mingchun
and Deng, Wei and Maza, Elie
and Mila, Isabelle
and Hu, Guojian
and Zouine, Mohamed
and Frasse, Pierre
and Vrebalov, Julia T and Giovannoni, James J and Li, Zhengguo and Van der Rest, Benoît
and Bouzayen, Mondher
Overexpression of the class D MADS-box gene Sl-AGL11 impacts fleshy tissue differentiation and structure in tomato fruits.
(2017)
Journal of Experimental Botany, 68 (17). 4869-4884. ISSN 0022-0957
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(Document in English)
PDF (Author's version) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader 1MB |
Official URL: http://dx.doi.org/10.1093/jxb/erx303
Abstract
MADS-box transcription factors are key elements of the genetic networks controlling flower and fruit development. Among these, the class D clade gathers AGAMOUS-like genes which are involved in seed, ovule, and funiculus development. The tomato genome comprises two class D genes, Sl-AGL11 and Sl-MBP3 , both displaying high expression levels in seeds and in central tissues of young fruits. The potential effects of Sl-AGL11 on fruit development were addressed through RNAi silencing and ectopic expression strategies. Sl-AGL11-down-regulated tomato lines failed to show obvious phenotypes except a slight reduction in seed size. In contrast, Sl-AGL11 overexpression triggered dramatic modifications of flower and fruit structure that include: the conversion of sepals into fleshy organs undergoing ethylene-dependent ripening, a placenta hypertrophy to the detriment of locular space, starch and sugar accumulation, and an extreme softening that occurs well before the onset of ripening. RNA-Seq transcriptomic profiling high-lighted substantial metabolic reprogramming occurring in sepals and fruits, with major impacts on cell wall-related genes. While several Sl-AGL11-related phenotypes are reminiscent of class C MADS-box genes (TAG1 and TAGL1), the modifications observed on the placenta and cell wall and the Sl-AGL11 expression pattern suggest an action of this class D MADS-box factor on early fleshy fruit development.
Item Type: | Article |
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Additional Information: | Thanks to Oxford University Press. The definitive version of this document is available at: https://academic.oup.com/jxb/article-abstract/68/17/4869/4157867 |
HAL Id: | hal-01643490 |
Audience (journal): | International peer-reviewed journal |
Uncontrolled Keywords: | |
Institution: | Other partners > Chongqing University (CHINA) Other partners > Cornell University (USA) Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE) French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE) |
Laboratory name: | |
Funders: | the Labex TULIP - the TomGEM H2020 project - the National Key Research and Development Program - the National Natural Science Foundation of China - L. Lemonnier - D. Saint-Martin |
Statistics: | download |
Deposited On: | 06 Nov 2017 12:45 |
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