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Overexpression of the class D MADS-box gene Sl-AGL11 impacts fleshy tissue differentiation and structure in tomato fruits

Huang, Baowen and Routaboul, Jean-Marc and Liu, Mingchun and Deng, Wei and Maza, Elie and Mila, Isabelle and Hu, Guojian and Zouine, Mohamed and Frasse, Pierre and Vrebalov, Julia T and Giovannoni, James J and Li, Zhengguo and Van der Rest, Benoît and Bouzayen, Mondher Overexpression of the class D MADS-box gene Sl-AGL11 impacts fleshy tissue differentiation and structure in tomato fruits. (2017) Journal of Experimental Botany, 68 (17). 4869-4884. ISSN 0022-0957

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Official URL: http://dx.doi.org/10.1093/jxb/erx303

Abstract

MADS-box transcription factors are key elements of the genetic networks controlling flower and fruit development. Among these, the class D clade gathers AGAMOUS-like genes which are involved in seed, ovule, and funiculus development. The tomato genome comprises two class D genes, Sl-AGL11 and Sl-MBP3 , both displaying high expression levels in seeds and in central tissues of young fruits. The potential effects of Sl-AGL11 on fruit development were addressed through RNAi silencing and ectopic expression strategies. Sl-AGL11-down-regulated tomato lines failed to show obvious phenotypes except a slight reduction in seed size. In contrast, Sl-AGL11 overexpression triggered dramatic modifications of flower and fruit structure that include: the conversion of sepals into fleshy organs undergoing ethylene-dependent ripening, a placenta hypertrophy to the detriment of locular space, starch and sugar accumulation, and an extreme softening that occurs well before the onset of ripening. RNA-Seq transcriptomic profiling high-lighted substantial metabolic reprogramming occurring in sepals and fruits, with major impacts on cell wall-related genes. While several Sl-AGL11-related phenotypes are reminiscent of class C MADS-box genes (TAG1 and TAGL1), the modifications observed on the placenta and cell wall and the Sl-AGL11 expression pattern suggest an action of this class D MADS-box factor on early fleshy fruit development.

Item Type:Article
Additional Information:Thanks to Oxford University Press. The definitive version of this document is available at: https://academic.oup.com/jxb/article-abstract/68/17/4869/4157867
HAL Id:hal-01643490
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Other partners > Chongqing University (CHINA)
Other partners > Cornell University (USA)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Laboratory name:
Funders:
the Labex TULIP - the TomGEM H2020 project - the National Key Research and Development Program - the National Natural Science Foundation of China - L. Lemonnier - D. Saint-Martin
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Deposited By: Brigitte LAFFORGUE
Deposited On:06 Nov 2017 12:45

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