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Using in-situ viscosimetry and morphogranulometry to explore hydrolysis mechanisms of filter paper and pretreated sugarcane bagasse under semi-dilute suspensions

Le, Tuan and Anne-Archard, Dominique and Coma, Véronique and Cameleyre, Xavier and Lombard, Eric and To, Kim Anh and Pham, Tuan Anh and Nguyen, Tien Cuong and Fillaudeau, Luc Using in-situ viscosimetry and morphogranulometry to explore hydrolysis mechanisms of filter paper and pretreated sugarcane bagasse under semi-dilute suspensions. (2017) Biochemical Engineering Journal, 127. 9-20. ISSN 1369-703X

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Official URL: http://dx.doi.org/10.1016/j.bej.2017.07.006


The relationship between in-situ viscosimetry, in- and ex-situ morphogranulometry, and biochemistry has been investigated during enzymatic hydrolysis of pretreated sugarcane bagasse (SCB, 3% w/v) and filter paper (FP, 1.5% w/v) at various enzyme dosages (0.3–25 FPU/g cellulose). Semi-dilute conditions (1.5–2 times higher than critical concentrations) were considered in order to generate non-Newtonian rheological behaviors and to avoid neglecting particle–particle interactions in opposition to dilute conditions. Observed phenomena as well as hydrolysis mechanisms including solvation, separation, fragmentation, and solubilization appeared to depend strongly on initial substrate properties. For both FP and SCB, suspension viscosities were correlated with particle size distributions and volume fractions during hydrolysis, but they exhibited strongly different trends. With SCB, a viscosity overtaking was clearly observed at enzyme loading ≤10 FPU/g cellulose. This phenomenon is explained by the separation of agglomerates into individual fragments, leading to an increase in the total number of particles and a morphological shift of particles from sphere-like into fiber-like. With FP, the viscosity collapsed at the initial stage in relation to the volume reduction of coarse particles and the number increase of fine particles. Suspension viscosity was strongly dependent on the fraction of coarse population and nearly independent from the glucose conversion yield.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The original PDF of the article can be found at http://www.sciencedirect.com/science/article/pii/S1369703X17301869
HAL Id:hal-01611335
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Other partners > Ecole Nationale Supérieure de Chimie et de Physique de Bordeaux - ENSCPB (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Université de Toulouse > Institut National des Sciences Appliquées de Toulouse - INSA (FRANCE)
Other partners > Institut Polytechnique de Bordeaux - IPB (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Other partners > Université des Sciences et des Technologies de Hanoi - USTH (VIETNAM)
Other partners > Université de Bordeaux (FRANCE)
Laboratory name:
Deposited On:21 Sep 2017 13:55

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