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Effects of chilling on the expression of ethylene biosynthetic genes in Passe-Crassane pear (Pyrus communis L.) fruits.

Lelièvre, Jean-Marc and Tichit, Line and Dao, Patrick and Fillion, Laurent and Nam, Young-Woo and Pech, Jean-Claude and Latché, Alain Effects of chilling on the expression of ethylene biosynthetic genes in Passe-Crassane pear (Pyrus communis L.) fruits. (1997) Plant Molecular Biology, 3 (5). 847-855. ISSN 0167-4412

(Document in English)

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Official URL: http://dx.doi.org/10.1023/A:1005750324531


Passe-Crassane pears require a 3-month chilling treatment at 0 C to be able to produce ethylene and ripen autonomously after subsequent rewarming. The chilling treatment strongly stimulated ACC oxidase activity, and to a lesser extent ACC synthase activity. At the same time, the levels of mRNAs hybridizing to ACC synthase and ACC oxidase probes increased dramatically. Fruit stored at 18 C immediately after harvest did not exhibit any of these changes, while fruit that had been previously chilled exhibited a burst of ethylene production associated with high activity of ACC oxidase and ACC synthase upon rewarming. ACC oxidase mRNA strongly accumulated in rewarmed fruits, while ACC synthase mRNA level decreased. The chilling-induced accumulation of ACC synthase and ACC oxidase transcripts was strongly reduced when ethylene action was blocked during chilling with 1-methylcyclopropene (1-MCP). Upon rewarming ACC synthase and ACC oxidase transcripts rapidly disappeared in 1-MCP-treated fruits. A five-week treatment of non-chilled fruits with the ethylene analog propylene led to increased expression of ACC oxidase and to ripening. However, ethylene synthesis, ACC synthase activity and ACC synthasemRNAs remained at very lowlevel. Our data indicate thatACC synthase gene expression is regulated by ethylene only during, or after chilling treatment, while ACC oxidase gene expression can be induced separately by either chilling or ethylene.

Item Type:Article
Additional Information:Thanks to Kluwer editor. The definitive version is available at http://www.springerlink.com The original PDF of the article can be found at Plant Molecular Biology website : http://www.springerlink.com/content/jp08561077t37065/
HAL Id:hal-03607936
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Laboratory name:
Deposited On:16 Dec 2008 10:30

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