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Thermal and vibrational characterization of human skin

Tang, Rong and Samouillan, Valérie and Dandurand, Jany and Lacabanne, Colette and Nadal-Wollbold, Florence and Casas, Christiane and Schmitt, Anne-Marie Thermal and vibrational characterization of human skin. (2017) Journal of Thermal Analysis and Calorimetry, 127 (2). 1143-1154. ISSN 1388-6150

(Document in English)

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Official URL: http://dx.doi.org/10.1007/s10973-016-5384-z


For a better understanding of the molecular and organizational changes in human dermis, biophysical methods were tested. The aim of this study was to find suitable and reproducible biomarkers for further clinical studies on intrinsic and extrinsic aging of dermis. Thermoporometry, hydric organization and thermal transitions of fresh and frozen skins were determined by differential scanning calorimetry (DSC). Fourier transform infrared spectroscopy (FTIR) was used to identify the absorption bands of the dermis especially in the 1800–1000 cm−1 zone and to discriminate between the different secondary structures of proteins. A widening of the pore size distribution is evidenced with freezing, but there is no significant difference between the hydric organization and the endothermic collagen denaturation of fresh and frozen skins. The global FTIR spectra and the second derivative spectra in the scanned zone are also identical in fresh and frozen dermis, validating the storage protocol. DSC and FTIR are well-suited techniques to characterize human skin, giving accurate results with high reproducibility. The acquisition of thermal and vibrational biomarkers of the skin at the mesoscale and nanoscale contributes to its better knowledge and is promising for further studies on skin aging.

Item Type:Article
Additional Information:Thanks to Springer editor. The definitive version is available at https://link.springer.com/ The original PDF of the article can be found at https://link.springer.com/article/10.1007/s10973-016-5384-z
HAL Id:hal-01536722
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Other partners > Laboratoires Pierre Fabre (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Laboratory name:
Deposited On:12 Jun 2017 09:04

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