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Grapevine Rootstocks Differentially Affect the Rate of Ripening and Modulate Auxin-Related Genes in Cabernet Sauvignon Berries

Corso, Massimiliano and Vannozzi, Alessandro and Ziliotto, Fiorenza and Zouine, Mohamed and Maza, Elie and Nicolato, Tommaso and Vitulo, Nicola and Meggio, Franco and Valle, Giorgio and Bouzayen, Mondher and Müller, Maren and Munné-Bosch, Sergi and Lucchin, Margherita and Bonghi, Claudio Grapevine Rootstocks Differentially Affect the Rate of Ripening and Modulate Auxin-Related Genes in Cabernet Sauvignon Berries. (2016) Frontiers in Plant Science, 7. 1-14. ISSN 1664-462X

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Official URL: http://dx.doi.org/10.3389/fpls.2016.00069

Abstract

In modern viticulture, grafting commercial grapevine varieties on interspecific rootstocks is a common practice required for conferring resistance to many biotic and abiotic stresses. Nevertheless, the use of rootstocks to gain these essential traits is also known to impact grape berry development and quality, although the underlying mechanisms are still poorly understood. In grape berries, the onset of ripening (véraison) is regulated by a complex network of mobile signals including hormones such as auxins, ethylene, abscisic acid, and brassinosteroids. Recently, a new rootstock, designated M4, was selected based on its enhanced tolerance to water stress and medium vigor. This study investigates the effect of M4 on Cabernet Sauvignon (CS) berry development in comparison to the commercial 1103P rootstock. Physical and biochemical parameters showed that the ripening rate of CS berries is faster when grafted onto M4. A multifactorial analysis performed on mRNA-Seq data obtained from skin and pulp of berries grown in both graft combinations revealed that genes controlling auxin action (ARF and Aux/IAA) represent one of main categories affected by the rootstock genotype. Considering that the level of auxin tightly regulates the transcription of these genes, we investigated the behavior of the main gene families involved in auxin biosynthesis and conjugation. Molecular and biochemical analyses confirmed a link between the rate of berry development and the modulation of auxin metabolism. Moreover, the data indicate that this phenomenon appears to be particularly pronounced in skin tissue in comparison to the flesh.

Item Type:Article
Additional Information:Thanks to Frontiers editor. The definitive version is available at http://journal.frontiersin.org/article/10.3389/fpls.2016.00069/full
HAL Id:hal-01520261
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Other partners > Universitat Autònoma de Barcelona - UAB (SPAIN)
Other partners > Università degli Studi di Padova (ITALY)
Laboratory name:
Funders:
AGER “SERRES”
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Deposited On:09 May 2017 12:43

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