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Bacteria transfer by deformation through microfiltration membrane

Gaveau, Arthur and Coetsier, Clémence and Roques, Christine and Bacchin, Patrice and Dague, Etienne and Causserand, Christel Bacteria transfer by deformation through microfiltration membrane. (2017) Journal of Membrane Science, 523. 446-455. ISSN 0376-7388

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Official URL: http://dx.doi.org/10.1016/j.memsci.2016.10.023


Living particles such as bacteria are able to transfer through membrane pores that are smaller than cell size due to the specific stiffness of this type of microorganism. This phenomenon can lead to a significant loss of selectivity in the filtration process, which is a major cause of concern in the sterilizing filtration step. This study investigates the retention of three bacteria strains: Escherichia coli CIP 54124, Pseudomonas aeruginosa CIP 103467 and Staphylococcus aureus CIP 53154 by model porous membranes for various operating conditions (transmembrane pressure, feed concentration and the physicochemical composition of filtered media with antibacterial agent added at sublethal concentration). The first part of this study is dedicated to defining the size and the nanomechanical properties of the envelope of the studied bacteria by microscopic techniques (Transmission electron microscopy & Atomic-force microscopy), in order to then explore the role of these quantifiable characteristics on the cell transfer through the pores by deformation mechanisms. Our results lead to the development of a numerical model to connect the observed retention efficiency of the filtration experiment and the microscopic information about individual particles.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com
HAL Id:hal-01451400
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Institut National des Sciences Appliquées de Toulouse - INSA (FRANCE)
Université de Toulouse > Institut Supérieur de l'Aéronautique et de l'Espace - ISAE-SUPAERO (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Université de Toulouse > Université Toulouse - Jean Jaurès - UT2J (FRANCE)
Laboratory name:
Region Midi-Pyrénées - FRBT
Deposited On:19 Dec 2016 10:14

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