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Effect of temperature on Brettanomyces bruxellensis: metabolic and kinetic aspects

Brandam, Cédric and Castro Martinez, Claudia and Délia, Marie-Line and Ramon Portugal, Felipe and Strehaiano, Pierre Effect of temperature on Brettanomyces bruxellensis: metabolic and kinetic aspects. (2008) Canadian Journal of Microbiology (CJM), 5 (1). 11-18. ISSN 1480-3275

(Document in English)

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Official URL: http://dx.doi.org/10.1139/W07-126


The effect of temperatures ranging from 15 to 35 °C on a culture of Brettanomyces bruxellensis was investigated in regards to thermodynamics, metabolism, and kinetics. In this temperature range, we observed an increase in growth and production rates. The growth behavior was well represented using the Arrhenius model, and an apparent activation energy of 16.61 kcal/mol was estimated. A stuck fermentation was observed at 35 °C as represented by high cell death. The carbon balance established that temperature had no effect on repartition of the glucose consumption between biomass and products. Hence, the same biomass concentration was obtained for all temperatures, except at 35 °C. Moreover, using logistic and Luedeking–Piret models, we demonstrated that production rates of ethanol and acetic acid were partially growth associated. Parameters associated with growth (αeth and αaa) remained constant with changing temperature, whereas, parameters associated with the population (βeth and βaa) varied. Optimal values were obtained at 32 °C for ethanol and at 25 °C for acetic acid.

Item Type:Article
Additional Information:Thanks to National Research Council Canada editor. The definitive version is available at http://pubs.nrc-cnrc.gc.ca The original PDF of the article can be found at Canadian Journal of Microbiology website : http://pubs.nrc-cnrc.gc.ca/rp-ps/journalDetail.jsp?jcode=cjm&lang=eng
HAL Id:hal-03591054
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Laboratory name:
Laboratoire de Génie Chimique - LGC (Toulouse, France) - Bioprocédés et systèmes microbiens (BioSyM)
Deposited On:20 Nov 2008 11:06

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