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Going beyond 20 μm-sized channels for studying red blood cell phase separation in microfluidic bifurcations

Roman, Sophie and Merlo, Adlan and Duru, Paul and Risso, Frédéric and Lorthois, Sylvie Going beyond 20 μm-sized channels for studying red blood cell phase separation in microfluidic bifurcations. (2016) Biomicrofluidics, 10 (3). 034103. ISSN 1932-1058

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Official URL: http://dx.doi.org/10.1063/1.4948955

Abstract

Despite the development of microfluidics, experimental challenges are considerable for achieving a quantitative study of phase separation, i.e., the non-proportional dis- tribution of Red Blood Cells (RBCs) and suspending fluid, in microfluidic bifurca- tions with channels smaller than 20lm. Yet, a basic understanding of phase separation in such small vessels is needed for understanding the coupling between microvascular network architecture and dynamics at larger scale. Here, we present the experimental methodologies and measurement techniques developed for that pur- pose for RBC concentrations (tube hematocrits) ranging between 2% and 20%. The maximal RBC velocity profile is directly measured by a temporal cross-correlation technique which enables to capture the RBC slip velocity at walls with high resolu- tion, highlighting two different regimes (flat and more blunted ones) as a function of RBC confinement. The tube hematocrit is independently measured by a photometric technique. The RBC and suspending fluid flow rates are then deduced assuming the velocity profile of a Newtonian fluid with no slip at walls for the latter. The accuracy of this combination of techniques is demonstrated by comparison with reference measurements and verification of RBC and suspending fluid mass conservation at individual bifurcations. The present methodologies are much more accurate, with less than 15% relative errors, than the ones used in previous in vivo experiments. Their potential for studying steady state phase separation is demonstrated, highlight- ing an unexpected decrease of phase separation with increasing hematocrit in symmetrical, but not asymmetrical, bifurcations and providing new reference data in regimes where in vitro results were previously lacking. Published by AIP Publishing

Item Type:Article
Additional Information:Thanks to AIP Publishing. The definitive version is available at http://scitation.aip.org The original PDF of the article can be found at Biomicrofluidics website : http://scitation.aip.org/content/aip/journal/bmf/10/3/10.1063/1.4948955
HAL Id:hal-01330390
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
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Funders:
European Commission - Institut National Polytechnique de Toulouse - INPT (France)
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Deposited On:10 Jun 2016 13:06

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