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Regionalization of browning revealed by whole subcutaneous adipose tissue imaging

Barreau, Corinne and Labit, Elodie and Guissard, Christophe and Rouquette, Jacques and Boizeau, Marie-Laure and Gani Koumassi, Souleymane and Carrière, Audrey and Jeanson, Yannick and Berger-Müller, Sandra and Dromard, Cécile and Plouraboué, Franck and Casteilla, Louis and Lorsignol, Anne Regionalization of browning revealed by whole subcutaneous adipose tissue imaging. (2016) Obesity, 00 (00). 1-9. ISSN 1930-7381

(Document in English)

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Official URL: http://dx.doi.org/10.1002/oby.21455


Objective: White and brown adipose tissues play a major role in the regulation of metabolic functions. With the explosion of obesity and metabolic disorders, the interest in adipocyte biology is growing constantly. While several studies have demonstrated functional differences between adipose fat pads, especially in their involvement in metabolic diseases, there are no data available on possible heterogeneity within an adipose depot. Methods: This study investigated the three-dimensional (3-D) organization of the inguinal fat pad in adult mice by combining adipose tissue clearing and autofluorescence signal acquisition by confocal microscopy. In addition, the study analyzed the expression of genes involved in adipocyte biology and browning at the mARN and protein levels in distinct areas of the inguinal adipose tissue, in control conditions and after cold exposure. Results: Semiautomated 3-D image analysis revealed an organization of the fat depot showing two regions: the core was structured into segmented lobules, whereas the periphery appeared unsegmented. Perilipin immunostaining showed that most of the adipocytes located in the core region had smaller lipid droplets, suggesting a brown-like phenotype. qPCR analysis showed a higher expression of the browning markers Ucp1, Prdm16, Ppargc1a, and Cidea in the core region than at the periphery. Finally, cold exposure induced upregulation of thermogenic gene expression associated with an increase of UCP1 protein, specifically in the core region of the inguinal fat depot. Conclusions: Altogether, these data demonstrate a structural and functional heterogeneity of the inguinal fat pad, with an anatomically restricted browning process in the core area.

Item Type:Article
Additional Information:Thanks to Wiley editor. The definitive version is available at http://onlinelibrary.wiley.com The original PDF of the article can be found at http://onlinelibrary.wiley.com/doi/10.1002/oby.21455/full
HAL Id:hal-01299930
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Other partners > Etablissement Français du Sang - EFS Midi-Pyrénées (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
French research institutions > Institut National de la Santé et de la Recherche Médicale - INSERM (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Laboratory name:
Deposited On:08 Apr 2016 13:13

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