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A TILLING allele of the tomato Aux/IAA9 gene offers new insights into fruit set mechanisms and perspectives for breeding seedless tomatoes

Mazzucato, Andrea and Cellini, Francesco and Bouzayen, Mondher and Zouine, Mohamed and Mila, Isabelle and Minoia, Silvia and Petrozza, Angelo and Picarella, Maurizio E. and Ruiu, Fabrizio and Carriero, Filomena A TILLING allele of the tomato Aux/IAA9 gene offers new insights into fruit set mechanisms and perspectives for breeding seedless tomatoes. (2015) Molecular Breeding, 35 (22). 1-15. ISSN 1380-3743

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Official URL: http://dx.doi.org/10.1007/s11032-015-0222-8

Abstract

Parthenocarpy is a desired trait in fruit crops; it enables fruit set under environmental conditions suboptimal for pollination, and seedless fruits represent a valuable consumer product. We employed TILLING-based screening of a mutant tomato population to find genetic lesions in Aux/IAA9, a negative regulator of the auxin response involved in the control of fruit set. We identified three mutations located in the coding region of this gene, including two singlebase substitutions and one single-base deletion, which leads to a frame shift and premature stop codon. The transcription of IAA9 was strongly reduced in the frame-shift mutant, and partial loss of mutated protein activity was evidenced by an in vitro transactivation assay. Whereas missense mutations were predicted to be tolerated and did not cause mutant phenotypes, the frame-shift mutation-induced phenotypes expected for a loss of IAA9 function, including altered axillary shoot growth, reduced leaf compoundness and a strong tendency to produce parthenocarpic fruits. Mutant flowers showed pleiotropic anther cone defects, a phenotype frequently associated with parthenocarpy in tomato and other species. Mutant fruits were larger than those of the seeded control, with higher brix

Item Type:Article
Additional Information:Thanks to Springer editor. The original PDF of the article can be found at Molecular Breeding website : http://link.springer.com/journal/11032
HAL Id:hal-01223244
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Other partners > Agenzia Lucana di Sviluppo e di Innovazione in Agricoltura - ALSIA (ITALY)
Other partners > Università degli Studi della Tuscia (ITALY)
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Deposited On:02 Nov 2015 11:35

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