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A DEMETER-like DNA demethylase governs tomato fruit ripening

Liu, Ruie and How-Kit, Alexandre and Stammitti, Linda and Teyssier, Emeline and Rolin, Dominique and Mortain-Bertrand, Anne and Halle, Stefanie and Liu, Mingchun and Kong, Junhua and Wu, Chaoqun and Degraeve-Guibault, Charlotte and Chapman, Natalie H. and Maucourt, Mickael and Hodgman, T. Charlie and Tost, Jörg and Bouzayen, Mondher and Hong, Yiguo and Seymour, Graham B. and Giovannoni, James J. and Gallusci, Philippe A DEMETER-like DNA demethylase governs tomato fruit ripening. (2015) Proceedings of the National Academy of Sciences, 112 (34). 10804-10809. ISSN 1091-6490

(Document in English)

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Official URL: http://dx.doi.org/10.1073/pnas.1503362112


In plants, genomic DNA methylation which contributes to development and stress responses can be actively removed by DEMETER-like DNA demethylases (DMLs). Indeed, in Arabidopsis DMLs are important for maternal imprinting and endosperm demethylation, but only a few studies demonstrate the developmental roles of active DNA demethylation conclusively in this plant. Here, we show a direct cause and effect relationship between active DNA demethylation mainly mediated by the tomato DML, SlDML2, and fruit ripening— an important developmental process unique to plants. RNAi SlDML2 knockdown results in ripening inhibition via hypermethylation and repression of the expression of genes encoding ripening transcription factors and rate-limiting enzymes of key biochemical processes such as carotenoid synthesis. Our data demonstrate that active DNA demethylation is central to the control of ripening in tomato.

Item Type:Article
Additional Information:Thanks to National Academy of Sciences editor. The definitive version is available at http://www.pnas.org/ The original PDF of the article can be found at PNAS website http://www.pnas.org/content/112/34/10804.full
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Commissariat à l'Energie Atomique et aux énergies alternatives - CEA (FRANCE)
Other partners > Cornell University (USA)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Other partners > University of Nottingham (UNITED KINGDOM)
Other partners > University of Warwick (UNITED KINGDOM)
Other partners > Fondation Jean Dausset - Centre d’Etude du Polymorphisme Humain - CEPH (FRANCE)
Other partners > Hangzhou Normal University - HZNU (CHINA)
Other partners > Université de Bordeaux (FRANCE)
Laboratory name:
Deposited On:05 Nov 2015 08:52

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