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Hydrolysis of concentrated lignocellulose suspensions with a cumulative feeding strategy based on a critical concentration : from laboratory to pilot scale

Nguyen, Tien-Cuong and Anne-Archard, Dominique and Coma, Véronique and Pichavant, Frédérique and Cameleyre, Xavier and Lombard, Eric and To, Kim Anh and Fillaudeau, Luc Hydrolysis of concentrated lignocellulose suspensions with a cumulative feeding strategy based on a critical concentration : from laboratory to pilot scale. (2015) In: Third European Congress of Applied Biotechnology - ECAB3, 27 September 2015 - 1 October 2015 (Nice, France). (Unpublished)

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In the context of biofuels and chemical production of petroleum substitutes from renewable carbon, bioconversion of lignocellulose biomasses is currently a major challenge. The limited knowledge of liquefaction and saccharification mechanisms stands as the main factor which penalizes bio-refinery progress. The objective of the present work was to increase the substrate concentration during hydrolysis in controlling transfer limitations by a specific strategy of substrate feeding. This concept was studied at laboratory scale and transposed into pilot scale. In-situ and ex-situ rheometry, morpho-granulometry and biochemical measurements were used to investigate transfer limitations. In a preliminary step (lab scale), rheological behaviour was modelled and a critical concentration (Ccrit ) inducing a sharp increase of viscosity was identified for Whatman paper (WP, 35 gdm.L-1) and paper pulp (PP, 31 gdm.L-1 ) [1]. Analysing hydrolysis experiments leads to assume an optimal feed rate Q* linked to this critical concentration. In a first step, cumulative feeding strategies (up to 10%w/w) were conducted for WP and PP in 2L reactor with different ratios Q/Q*. A cumulative feeding substrate strategy allows to considerably reduce the transfer limitations linked to high concentrations and to control the glucose production kinetics [2]. In the second step, the enzymatic hydrolysis (up to 20%w/w) was investigated in a 20L reactor coupled with an extruder (substrate feeding). This transposition will be discussed regarding the biochemical kinetics and physical limitations.

Item Type:Conference or Workshop Item (Lecture)
Audience (conference):International conference without published proceedings
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Université de Toulouse > Institut National des Sciences Appliquées de Toulouse - INSA (FRANCE)
Other partners > Institut Polytechnique de Bordeaux - IPB (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Other partners > Université des Sciences et des Technologies de Hanoi - USTH (VIETNAM)
Other partners > Université de Bordeaux (FRANCE)
Laboratory name:
Deposited On:28 Oct 2015 12:51

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