Girardi, Cesar Luis and Bermudez, Kalina and Bernadac, Anne and Chaves, Ana
and Zouine, Mohamed
and Jaimes Miranda, Fabiola
and Bouzayen, Mondher
and Pech, Jean-Claude
and Latché, Alain
The mitochondrial elongation factor LeEF-Tsmt is regulated during tomato fruit ripening and upon wounding and ethylene treatment.
(2006)
Postharvest Biology and Technology, 4 (1). 1-7. ISSN 0925-5214
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(Document in English)
PDF (Author's version) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader 600kB |
Official URL: http://dx.doi.org/10.1016/j.postharvbio.2006.06.002
Abstract
A gene encoding an elongation factor LeEF-Tsmt that participates in the protein synthesis process in mitochondria shows strong expression in ripening fruit as compared to other organs. It is strongly up-regulated during the first stages of the ripening process in parallel with the climacteric rise in respiration. LeEF-Tsmt expression is stimulated by ethylene, wounding and high temperature but ethylene-insensitive mutants exhibit normal expression. Transgenic fruit have been generated in which LeEF-Tsmt has been constitutively up- and down-regulated. Surprisingly, altering the expression of the gene by genetic transformation with antisense and sense LeEF-Tsmt constructs did not affect the pattern of respiration and ethylene production during ripening and upon wounding. In addition, expression of the alternative oxidase gene which is known to play an important role in respiratory climacteric was not affected. Possible reasons for the absence of effect on respiration of variations of LeEF-Tsmt gene expression are discussed.
Item Type: | Article |
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Additional Information: | Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com. The original PDF of the article can be found at Postharvest Biology and Technology website : http://www.sciencedirect.com/science/journal/09255214 |
HAL Id: | hal-03597983 |
Audience (journal): | International peer-reviewed journal |
Uncontrolled Keywords: | |
Institution: | French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE) Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE) |
Laboratory name: | |
Statistics: | download |
Deposited On: | 02 Dec 2008 09:32 |
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