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Tomato EF-Tsmt, a functional mitochondrial translation elongation factor from higher plants

Benichou, Mohamed and Li, Zhengguo and Tournier, Barthélémy and Chaves, Ana and Zegzouti, Hicham and Jauneau, Alain and Delalande, Corinne and Latché, Alain and Bouzayen, Mondher and Spremulli, Linda L. and Pech, Jean-Claude Tomato EF-Tsmt, a functional mitochondrial translation elongation factor from higher plants. (2003) Plant Molecular Biology, 5 (3). 411-422. ISSN 1573-5028

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Official URL: http://dx.doi.org/10.1023/B:PLAN.0000006943.23747.7d


Ethylene-induced ripening in tomato (Lycopersicon esculentum) resulted in the accumulation of a transcript designated LeEF-Tsmt that encodes a protein with significant homology to bacterial Ts translational elongation factor (EF-Ts). Transient expression in tobacco and sunflower protoplasts of full-length and truncated LeEF-Tsmt- GFP fusion constructs and confocal microscopy observations clearly demonstrated the targeting of LeEF-Tsmt to mitochondria and not to chloroplasts and the requirement for a signal peptide for the proper sorting of the protein. Escherichia coli recombinant LeEF-Tsmt co-eluted from Ni-NTA resins with a protein corresponding to the molecular weight of the elongation factor EF-Tu of E. coli, indicating an interaction with bacterial EF-Tu. Increasing the GDP concentration in the extraction buffer reduced the amount of EF-Tu in the purified LeEF-Tsmt fraction. The purified LeEF-Tsmt stimulated the poly(U)-directed polymerization of phenylalanine 10-fold in the presence of EF-Tu. Furthermore, LeEF-Tsmt was capable of catalysing the nucleotide exchange reaction with E. coli EF-Tu. Altogether, these data demonstrate that LeEF-Tsmt encodes a functional mitochondrial EF-Ts. LeEFTsmt represents the first mitochondrial elongation factor to be isolated and functionally characterized in higher plants.

Item Type:Article
Additional Information:Thanks to Springer editor. The original publication is available at www.springerlink.com. The original PDF of the article can be found at Plant Molecular Biology website : http://www.springerlink.com/content/h2611532862020m3/
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Other partners > University of Cadi Ayyad - UCAM (MOROCCO)
Other partners > North Carolina State University (USA)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Laboratory name:
Deposited On:02 Dec 2008 08:27

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