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A single-molecule approach to explore binding, uptake and transport of cancer cell targeting nanotubes

Lamprecht, Constanze and Plochberger, Birgit and Ruprecht, Verena and Wieser, Stefan and Rankl, Christian and Heister, Elena and Unterauer, Barbara and Brameshuber, Mario and Danzberger, Jürgen and Lukanov, Petar and Flahaut, Emmanuel and Schütz, Gerhard and Hinterdorfer, Peter and Ebner, Andreas A single-molecule approach to explore binding, uptake and transport of cancer cell targeting nanotubes. (2014) Nanotechnology, 25 (12). 125704. ISSN 0957-4484

(Document in English)

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Official URL: http://dx.doi.org/10.1088/0957-4484/25/12/125704


In the past decade carbon nanotubes (CNTs) have been widely studied as a potential drug-delivery system, especially with functionality for cellular targeting. Yet, little is known about the actual process of docking to cell receptors and transport dynamics after internalization. Here we performed single-particle studies of folic acid (FA) mediated CNT binding to human carcinoma cells and their transport inside the cytosol. In particular, we employed molecular recognition force spectroscopy, an atomic force microscopy based method, to visualize and quantify docking of FA functionalized CNTs to FA binding receptors in terms of binding probability and binding force. We then traced individual fluorescently labeled, FA functionalized CNTs after specific uptake, and created a dynamic 'roadmap' that clearly showed trajectories of directed diffusion and areas of nanotube confinement in the cytosol. Our results demonstrate the potential of a single-molecule approach for investigation of drug-delivery vehicles and their targeting capacity.

Item Type:Article
Additional Information:Thanks to IOP Publishing. The definitive version is available at http://iopscience.iop.org The original PDF of the article can be found at Nanotechnology website : http://iopscience.iop.org/0957-4484/25/12/125704/
HAL Id:hal-01132418
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Other partners > Technische Universität Wien - TU Wien (AUSTRIA)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Other partners > Agilent Technologies (USA)
Other partners > Center for Advanced Bioanalysis - CBL (AUSTRIA)
Other partners > Christian-Albrechts-Universität zu Kiel - CAU (GERMANY)
Other partners > Institute of Science and Technology Austria - IST Austria (AUSTRIA)
Other partners > Linz Johannes Kepler University (AUSTRIA)
Other partners > University of Surrey (UNITED KINGDOM)
Laboratory name:
Deposited On:12 Mar 2015 16:25

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