Joannis-Cassan, Claire and Délia, Marie-Line and Riba, Jean-Pierre
Biofilm growth kinetics on hydrocarbon in a porous medium under biostimulation conditions.
(2007)
Environmental Progress, 26 (2). 140-148. ISSN 1547-5921
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(Document in English)
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Official URL: http://dx.doi.org/10.1002/ep.10189
Abstract
The formation of biofilm on hydrocarbon in a porous medium under biostimulation conditions was investigated in order to a better understanding of microbial development during bioremediation of hydrocarbon-contaminated soils. Pseudomonas alkanolytica ATCC 21034 was studied as it grows embedded in a biofilm on dodecane surface. First, an experimental protocol for determination of biofilm growth kinetics on hydrocarbon in a porous medium was proposed. Six identical columns packed with sand were run simultaneously. The growth kinetics were obtained by measuring the biofilm dry weight of each column at different times. The reproducibility of the experimental protocol was validated using statistical tests. Then biofilm growth kinetics were established in the porous medium for two specific surfaces of dodecane (large or small droplets). A linear growth was observed due to transport limitation of both dodecane and dissolved oxygen in the biofilm. So the degradation of dodecane was uncompleted. Moreover, difficulty to bring dissolved oxygen in the deeper part of the porous medium was pointed out. All these phenomena could explain some failures encountered during biostimulation processes.
Item Type: | Article |
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Audience (journal): | International peer-reviewed journal |
Uncontrolled Keywords: | |
Institution: | French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE) Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE) Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE) Other partners > Ecole Centrale Paris (FRANCE) |
Laboratory name: | Laboratoire de Génie Chimique - LGC (Toulouse, France) - Bioprocédés et systèmes microbiens (BioSyM) Laboratoire de Génie des Procédés et Matériaux- LGPM (Châtenay-Malabry, France) |
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Deposited On: | 28 Oct 2008 13:04 |
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