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Antimicrobial compounds produced by Actinomadura sp. AC104 isolated from an Algerian Saharan soil

Badji, Boubekeur and Zitouni, Abdelghani and Mathieu, Florence and Lebrihi, Ahmed and Sabaou, Nasserdine Antimicrobial compounds produced by Actinomadura sp. AC104 isolated from an Algerian Saharan soil. (2006) Canadian Journal of Microbiology, 5 (4). 373-382. ISSN 1480-3275

(Document in English)

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Official URL: http://dx.doi.org/10.1139/W05-132


During a search for nonpolyenic antifungal antibiotics, an actinomycete designated AC104 was isolated from a Saharan soil sample by a dilution agar plating method using a chitin – vitamins B medium supplemented with rifampicin. Isolate AC104 presented the morphological and the chemical characteristics of the genus Actinomadura. On the basis of 76 physiological tests and 16S rDNA analysis, this isolate was determined to be quite different from the known species of Actinomadura. It is active against filamentous fungi and both Gram-positive and Gram-negative bacteria. The production of antibiotic substances was investigated using several culture media. The highest antimicrobial activities were obtained on ISP2 medium. The benzenic extract contained five bioactive spots detected on thin layer chromatography plates. Among these antibiotics, a complex called 104A, which showed the more interesting antifungal activity, was selected and purified by reverse-phase high-pressure liquid chromatography. This complex is composed of four compounds. Ultraviolet-visible, infrared, mass, and 1H nuclear magnetic resonance spectroscopy studies showed that these molecules contain an aromatic ring substituted by aliphatic chains. These compounds differ from the known antibiotics produced by Actinomadura species.

Item Type:Article
Additional Information:Thanks to National Research Council Canada editor. The definitive version is available at http://pubs.nrc-cnrc.gc.ca The original PDF of the article can be found at Canadian Journal of Microbiology website : http://pubs.nrc-cnrc.gc.ca/rp-ps/journalDetail.jsp?jcode=cjm&lang=eng
HAL Id:hal-03598045
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Other partners > Ecole normale supérieure de Kouba (ALGERIA)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Other partners > Centre de Recherche Scientifique et Technique sur les Régions Arides - CRSTRA (ALGERIA)
French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Laboratory name:
Deposited On:15 Oct 2008 12:49

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