OATAO - Open Archive Toulouse Archive Ouverte Open Access Week

The twin-screw extrusion technology, an original solution for the extraction of proteins from sunflower and alfalfa

Evon, Philippe and Colas, Dorothée and Pontalier, Pierre-Yves and Rigal, Luc The twin-screw extrusion technology, an original solution for the extraction of proteins from sunflower and alfalfa. (2010) In: 101st AOCS Annual Meeting & Expo, 16 May 2010 - 19 May 2010 (Phoenix, Arizona, United States). (Unpublished)

(Document in English)

PDF (Author's version) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader


Twin-screw extrusion has been used for the protein extraction from sunflower and alfalfa. Thermo-mechanical fractionation and aqueous extraction are conducted simultaneously to collect separately a liquid extract and a solid raffinate. From sunflower whole plant, squeezing in the reversed screws is favored by the fibers abundance in the stalk, and it enables L/S separation. Protein yield is 44%, in the best conditions, and lipids are partly co-extracted. Water-soluble proteins are in an aqueous extract and in two O/W emulsions due to their surface-active properties. Hence, the oil is co-extracted in the form of emulsions stabilized by proteins at interface. Proteins can be collected in the aqueous extract by isoelectric precipitation. Emulsions are usable for oil production. Their demulsification with ethanol produces a precipitate rich in proteins with low denaturation level. From alfalfa whole plant, the highest protein yield in the aqueous extract (called green juice) is 25% when water is added in the extruder. Water-soluble and water-insoluble green juice proteins can be fractionated thanks to L/L extraction in sunflower oil and ammonium sulfate precipitation. Some other molecules are co-extracted (polysaccharides, phenolic compounds). They can be purified with ultrafiltration or chromatography.

Item Type:Invited Conference
HAL Id:hal-04026577
Audience (conference):International conference without published proceedings
Uncontrolled Keywords:
Institution:Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Laboratory name:
Deposited On:05 Sep 2014 11:37

Repository Staff Only: item control page