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The chimeric repressor version of an Ethylene Response Factor(ERF) family member, Sl-ERF.B3, shows contrasting effects on tomato fruit ripening

Liu, Mingchun and Diretto, Gianfranco and Pirrello, Julien and Roustan, Jean-Paul and Li, Zhengguo and Giuliano, Giovanni and Regad, Farid and Bouzayen, Mondher The chimeric repressor version of an Ethylene Response Factor(ERF) family member, Sl-ERF.B3, shows contrasting effects on tomato fruit ripening. (2014) New Phytologist, 203 (1). 206-218. ISSN 0028-646X

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Official URL: http://dx.doi.org/10.1111/nph.12771


Fruit ripening involves a complex interplay between ethylene and ripening-associated transcriptional regulators. Ethylene Response Factors (ERFs) are downstream components of ethylene signaling, known to regulate the expression of ethylene-responsive genes. Although fruit ripening is an ethylene-regulated process, the role of ERFs remains poorly understood. The role of Sl-ERF.B3 in tomato (Solanum lycopersicum) fruit maturation and ripening is addressed here using a chimeric dominant repressor version (ERF.B3-SRDX). Over-expression of ERF.B3-SRDX results in a dramatic delay of the onset of ripening, enhanced climacteric ethylene production and fruit softening, and reduced pigment accumulation. Consistently, genes involved in ethylene biosynthesis and in softening are up-regulated and those of carotenoid biosynthesis are down-regulated. Moreover, the expression of ripening regulators, such as RIN, NOR, CNR and HB-1, is stimulated in ERF.B3-SRDX dominant repressor fruits and the expression pattern of a number of ERFs is severely altered. The data suggest the existence of a complex network enabling interconnection between ERF genes which may account for the pleiotropic alterations in fruit maturation and² ripening. Overall, the study sheds new light on the role of Sl-ERF.B3 in the transcriptional network controlling the ripening process and uncovers a means towards uncoupling some of the main ripening-associated processes.

Item Type:Article
Additional Information:Thanks to Wiley editor. The definitive version is available at http://onlinelibrary.wiley.com/doi/10.1111/nph.12771/abstract;jsessionid=8DE2285488F71DBD3AB26307683379D7.f02t03
HAL Id:hal-02632475
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Other partners > Chongqing University (CHINA)
Other partners > Italian National Agency for New Technologies, Energy and Environment - ENEA (ITALY)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Laboratory name:
Deposited On:22 Apr 2014 08:15

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