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Expression of auxin-binding protein1 during plum fruit ontogeny supports the potential role of auxin in initiating and enhancing climacteric ripening

El-Sharkawy, Islam and Sherif, Sherif and Mahboob, Abdullah and Abubaker, Kamal and Bouzayen, Mondher and Jayasankar, Subramanian Expression of auxin-binding protein1 during plum fruit ontogeny supports the potential role of auxin in initiating and enhancing climacteric ripening. (2012) Plant Cell Reports, 31 (10). 1911-1921. ISSN 0721-7714

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Official URL: http://dx.doi.org/10.1007/s00299-012-1304-2

Abstract

Auxin-binding protein1 (ABP1) is an active element involved in auxin signaling and plays critical roles in auxin-mediated plant development. Here, we report the isolation and characterization of a putative sequence from Prunus salicina L., designated PslABP1. The expected protein exhibits a similar molecular structure to that of well-characterized maize-ABP1; however, PslABP1 displays more sequence polarity in the active-binding site due to substitution of some crucial amino-acid residues predicted to be involved in auxin-binding. Further, PslABP1 expression was assessed throughout fruit ontogeny to determine its role in fruit development. Comparing the expression data with the physiological aspects that characterize fruit-development stages indicates that PslABP1 up-regulation is usually associated with the signature events that are triggered in an auxin-dependent manner such as floral induction, fruit initiation, embryogenesis, and cell division and elongation. However, the diversity in PslABP1 expression profile during the ripening process of early and late plum cultivars seems to be due to the variability of endogenous auxin levels among the two cultivars, which consequently can change the levels of autocatalytic ethylene available for the fruit to co-ordinate ripening. The effect of auxin on stimulating ethylene production and in regulating PslABP1 was investigated. Our data suggest that auxin is involved in the transition of the mature green fruit into the ripening phase and in enhancing the ripening process in both auxin- and ethylene-dependent manners thereafter.

Item Type:Article
Additional Information:Thanks to Springer editor. The definitive version is available athttp://link.springer.com/article/10.1007%2Fs00299-012-1304-2
HAL Id:hal-00979256
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Other partners > Damanhour University (EGYPT)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Other partners > Brock University (CANADA)
Other partners > University of Guelph (CANADA)
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Deposited By: Brigitte LAFFORGUE
Deposited On:15 Apr 2014 14:05

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