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Leaf-associated fungal diversity in acidified streams: insights from combining traditional and molecular approaches

Clivot, Hugues and Cornut, Julien and Chauvet, Eric and Elger, Arnaud and Poupin, Pascal and Guérold, François and Pagnout, Christophe Leaf-associated fungal diversity in acidified streams: insights from combining traditional and molecular approaches. (2014) Environmental Microbiology, 16 (7). 2145-2156. ISSN 1462-2912

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Official URL: http://dx.doi.org/10.1111/1462-2920.12245


We combined microscopic and molecular methods to investigate fungal assemblages on alder leaf litter exposed in the benthic and hyporheic zones of five streams across a gradient of increasing acidification for 4 weeks. The results showed that acidification and elevated Al concentrations strongly depressed sporulating aquatic hyphomycetes diversity in both zones of streams, while fungal diversity assessed by denaturing gradient gel electrophoresis (DGGE) appeared unaffected. Clone library analyses revealed that fungal communities on leaves were dominated by members of Ascomycetes and to a lesser extent by Basidiomycetes and Chytridiomycetes. An important contribution of terrestrial fungi was observed in both zones of the most acidified stream and in the hyporheic zone of the reference circumneutral stream. The highest leaf breakdown rate was observed in the circumneutral stream and occurred in the presence of both the highest diversity of sporulating aquatic hyphomycetes and the highest contribution to clone libraries of sequences affiliated with aquatic hypho- mycetes. Both methods underline the major role played by aquatic hyphomycetes in leaf decom- position process. Our findings also bring out new highlights on the identity of leaf-associated fungal communities and their responses to anthropogenic alteration of running water ecosystems.

Item Type:Article
Additional Information:Thanks to Wiley Blackwell editor. The definitive version is available at http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1462-2920 The original PDF of the article can be found at Environmental Microbiology website : http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.12245/full
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Other partners > Université de Lorraine (FRANCE)
Laboratory name:
Deposited On:07 Mar 2014 07:53

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