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Optimization of pressurized liquid extraction using a multivariate chemometric approach and comparison of solid phase extraction cleanup steps for the determination of polycyclic aromatic hydrocarbons in mosses

Foan, Louise and Simon, Valérie Optimization of pressurized liquid extraction using a multivariate chemometric approach and comparison of solid phase extraction cleanup steps for the determination of polycyclic aromatic hydrocarbons in mosses. (2012) Journal of Chromatography A, 1256. 22-31. ISSN 0021-9673

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Official URL: http://dx.doi.org/10.1016/j.chroma.2012.07.065

Abstract

A factorial design was used to optimize the extraction of polycyclic aromatic hydrocarbons (PAHs) from mosses, plants used as biomonitors of air pollution. The analytical procedure consists of pressurized liquid extraction (PLE) followed by solid-phase extraction (SPE) cleanup, in association with analysis by high performance liquid chromatography coupled with fluorescence detection (HPLC-FLD). For method development, homogeneous samples were prepared with large quantities of the mosses Isothecium myosuroides Brid. and Hypnum cupressiforme Hedw., collected from a Spanish Nature Reserve. A factorial design was used to identify the optimal PLE operational conditions: 2 static cycles of 5 min at 80°C. The analytical procedure performed with PLE showed similar recoveries (∼70%) and total PAH concentrations (∼200 ng g-1) as found using Soxtec extraction, with the advantage of reducing solvent consumption by 3 (30 mL against 100 mL per sample), and taking a fifth of the time (24 samples extracted automatically in 8 h against 2 samples in 3.5 h). The performance of SPE normal phases (NH2, Florisil®, silica and activated aluminium) generally used for organic matrix cleanup was also compared. Florisil® appeared to be the most selective phase and ensured the highest PAH recoveries. The optimal analytical procedure was validated with a reference material and applied to moss samples from a remote Spanish site in order to determine spatial and inter-species variability.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The original PDF can be found at http://www.sciencedirect.com/science/article/pii/S0021967312011351
HAL Id:hal-00917566
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
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Deposited By: Valerie SIMON
Deposited On:12 Dec 2013 08:17

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