In-situ and ex-situ rheometry of high density Yarrowia lipolytica broth: determination of critical concentration and impact of yeastmycelial transition

Abstract

The specificity of microbial bioreactions which give rise to irreducible couplings with hydrodynamics and heat and mass transfers, led into complex (three phases medium) and dynamic (auto-biocatalytic reaction) systems. Cells (concentration, shape, dimension, physiology…) strongly affect physico-chemical properties of broth and the modification of these characteristics interacts with bioprocess performances (specific rates, yields…) with an improvement or, more generally, a decrease of yields.