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Freezing and chemical preservatives alter the stable isotope values of carbon and nitrogen of the Asiatic clam (Corbicula fluminea)

Syväranta, Jari and Martino, Aurelia and Kopp, Dorothée and Céréghino, Régis and Santoul, Frédéric Freezing and chemical preservatives alter the stable isotope values of carbon and nitrogen of the Asiatic clam (Corbicula fluminea). (2011) Hydrobiologia, 658 (1). 383-388. ISSN 0018-8158

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Official URL: http://dx.doi.org/10.1007/s10750-010-0512-4


We tested the impacts of most common sample preservation methods used for aquatic sample materials on the stable isotope ratios of carbon and nitrogen in clams, a typical baseline indicator organism for many aquatic food web studies utilising stable isotope analysis (SIA). In addition to common chemical preservatives ethanol and formalin, we also assessed the potential impacts of freezing on δ¹³C and δ¹⁵N values and compared the preserved samples against freshly dried and analysed samples. All preservation methods, including freezing, had significant impacts on δ¹³C and δ¹⁵N values and the effects in general were greater on the carbon isotope values (1.3-2.2% difference) than on the nitrogen isotope values (0.9-1.0% difference). However, the impacts produced by the preservation were rather consistent within each method during the whole 1 year experiment allowing these to be accounted for, if clams are intended for use in retrospective stable isotope studies.

Item Type:Article
Additional Information:Thanks to Springer verlag editor. The definitive version is avaialable at http://link.springer.com/article/10.1007%2Fs10750-010-0512-4
HAL Id:hal-00912536
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
French research institutions > Institut Français de Recherche pour l'Exploitation de la MER - IFREMER (FRANCE)
Other partners > Leibniz-Institut für Gewässerökologie und Binnenfischerei - IGB (GERMANY)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Laboratory name:
Deposited On:02 Dec 2013 11:03

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