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EFE multigene family in tomato plants: expression and characterization

Bouzayen, Mondher and Cooper, Wendy and Barry, Cornelius and Zegzouti, Hicham and Hamilton, Andrew J. and Grierson, Donald EFE multigene family in tomato plants: expression and characterization. (1993) In: Cellular and Molecular Aspects of the Plant Hormone Ethylene. Springer, Netherlands, 76-81. ISBN 978-94-017-1003-9

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Official URL: http://dx.doi.org/10.1007/978-94-017-1003-9_14

Abstract

Decisive progress in the understanding of ethylene metabolism was made when a cDNA clone (pTOM13) was identified by Hamilton et al. (1990) as encoding EFE. Previous screening of a tomato genomic library revealed three genomic clones, refered now to as ETH1, ETH2 and ETH3. The nucleotidic sequence of ETH3 is unknown while those of ETH1 and ETH2 have already been published. Moreover, ETH1 gene has been shown to encode pTOM 13 mRNA whereas no data were available so far concerning the expression of the two other genes. Following sequencing of ETH3 we report here the comparative analysis of the derived amino acid sequences encoded by this gene family and show that the three predicted proteins share the same hydrophilicity pattern. However, ETH2 protein displays significantly higher isoelectric point than the two others.We also describe the isolation and cloning of ETH2 cDNA and thus provides experimental evidence for its expression at the RNA level. Gene specific oligonucleotides were designed and used as primers in PCR reactions to amplify about 200 bp of the 3’ untranslated regions. The amplified fragments were cloned in a transcription vector generating appropriate probes for subsequent study of the expression of EFE genes in tomato plants.

Item Type:Book Section
Additional Information:Proceedings of the International Symposium on Cellular and Molecular Aspects of Biosynthesis and Action of the Plant Hormone Ethylene, Agen, France, August 31–September 4, 1992
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Institution:Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Other partners > University of Nottingham (UNITED KINGDOM)
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Deposited On:05 Mar 2014 15:09

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