OATAO - Open Archive Toulouse Archive Ouverte Open Access Week

Season's variation impact on Citrus aurantium leaves essential oil: chemical composition and biological activities

Ellouze, Inès and Abderrabba, Manef and Sabaou, Nasserdine and Mathieu, Florence and Lebrihi, Ahmed and Bouajila, Jalloul Season's variation impact on Citrus aurantium leaves essential oil: chemical composition and biological activities. (2012) Journal of Food Science, vol. 77 (n° 9). pp. T173-T180. ISSN 0022-1147

[img] (Document in English)

PDF (Author's version) - Depositor and staff only - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
8MB

Official URL: http://dx.doi.org/10.1111/j.1750-3841.2012.02846.x

Abstract

Citrus aurantium leaves' essential oils (EOs) were evaluated for chemical composition and antioxidant and antibacterial activities. The vegetable material, taken 5 times during the year, has undergone the hydrodistillation to prepare EO. Chemical characterization by gas chromatography/mass spectrometry and GC/flame ionization detection allowed the identification of 46 compounds, and a notable quantitative and qualitative differences between the different Petitgrain samples according to the harvest time. Linalool (43.2% to 65.97%), linalyl acetate (0.77% to 24.77%), and α-terpineol (9.29% to 12.12%) were the main components. The most important number of components was registered for summer EOs (July and September). The 5 EOs submitted biological activities screening, namely, antioxidant and antimicrobial activities. Weak antioxidant activities (IC50 values >10000 mg/L) were registered by both 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulphonate assays, mostly because the weak amount of phenols in EOs. Antibacterial activities (12 microorganisms) were registered against Gram-positive bacteria [Bacillus subtilis (MIC = 2.7 mg/mL), Staphylococcus aureus (4.8 mg/mL)], and moderated ones against yeasts [Saccharomyces cerevisiae (9.2 mg/mL)] and fungi [Mucor ramannianus (5 mg/mL)]. Positive correlations between the identified compounds and the antimicrobial activities were noted. Many compounds were correlated to antimicrobial activity mainly caryophyllene oxide against Escherichia coli (R² = 0.99), S. cerevisiae (R² = 0.99), and Fusarium culmorum (R² = 0.99).

Item Type:Article
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Other partners > Ecole normale supérieure de Kouba (ALGERIA)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS (FRANCE)
Other partners > Université de Carthage (TUNISIA)
Laboratory name:
Statistics:download
Deposited By: Vincent GERBAUD
Deposited On:31 Oct 2013 10:21

Repository Staff Only: item control page