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Actinopolyspora righensis sp. nov., a novel halophilic actinomycete isolated from Saharan soil in Algeria

Meklat, Atika and Bouras, Noureddine and Zitouni, Abdelghani and Mathieu, Florence and Lebrihi, Ahmed and Schumann, Peter and Spröer, Cathrin and Klenk, Hans-Peter and Sabaou, Nasserdine Actinopolyspora righensis sp. nov., a novel halophilic actinomycete isolated from Saharan soil in Algeria. (2013) Antonie van Leeuwenhoek, vol. 104 (n° 3). pp. 301-307. ISSN 0003-6072

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Official URL: http://dx.doi.org/10.1007/s10482-013-9948-7

Abstract

A novel halophilic actinomycete strain, H23T, was isolated from a Saharan soil sample collected in Djamaˆa (Oued Righ region), El-Oued province, South Algeria. Strain H23T was identified as a member of the genus Actinopolyspora by a polyphasic approach. Phylogenetic analysis showed that strain H23T had 16S rRNA gene sequence similarities ranging from 97.8 % (Actinopolyspora xinjiangensis TRM 40136T) to 94.8 % (Actinopolyspora mortivallis DSM 44261T). The strain grew optimally at pH 6.0–7.0, 28–32°C and in the presence of 15–25 % (w/v) NaCl. The substrate mycelium was well developed and fragmented with age. The aerialmyceliumproduced long, straight or flexuous spore chains with non-motile, smooth-surfaced and rod-shaped spores. Strain H23T had MK-10 (H4) and MK-9 (H4) as the predominant menaquinones. The whole microorganism hydrolysates mainly consisted of meso-diaminopimelic acid, galactose and arabinose. The diagnostic phospholipid detected was phosphatidylcholine. The major cellular fatty acids were anteiso-C17:0 (37.4 %), iso-C17:0 (14.8 %), iso-C15:0 (14.2 %), and iso-C16:0 (13.9 %). The genotypic and phenotypic data show that the strain represents a novel species of the genus Actinopolyspora, for which the name Actinopolyspora righensis sp. nov. is proposed, with the type strain H23T (=DSM 45501T = CCUG 63368T = MTCC 11562T).

Item Type:Article
Additional Information:Thanks to Springer Verlag editor. The definitive version is available at http://link.springer.com/article/10.1007%2Fs10482-013-9948-7
HAL Id:hal-01177618
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Other partners > Leibniz-Institut Deutsche Sammlung von Mikroorganismen und Zelllkulturen GmBH - DSMZ (GERMANY)
Other partners > Ecole normale supérieure de Kouba (ALGERIA)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > Université de Saâd Dahlab Blida - USDB (ALGERIA)
Other partners > Université Moulay Ismaïl de Meknès (MOROCCO)
Laboratory name:
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Deposited By: Vincent GERBAUD
Deposited On:22 Oct 2013 09:05

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