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Bifunctional silica nanoparticles for the exploration of biofilms of Pseudomonas aeruginosa

Mauline, Léïla and Gressier, Marie and Roques, Christine and Hammer, Peter and Ribeiro, Sidney J. L. and Caiut, José Maurício A. and Menu, Marie-Joëlle Bifunctional silica nanoparticles for the exploration of biofilms of Pseudomonas aeruginosa. (2013) Biofouling, vol. 29 (n ° 7). pp. 775-788. ISSN 0892-7014

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Official URL: http://dx.doi.org/10.1080/08927014.2013.798866

Abstract

Luminescent silica nanoparticles are frequently employed for biotechnology applications mainly because of their easy functionalization, photo-stability, and biocompatibility. Bifunctional silica nanoparticles (BSNPs) are described here as new efficient tools for investigating complex biological systems such as biofilms. Photoluminescence is brought about by the incorporation of a silylated ruthenium (II) complex. The surface properties of the silica particles were designed by reaction with amino-organosilanes, quaternary ammonium-organosilanes, carboxylate-organosilanes and hexamethyldisilazane. BSNPs were characterized extensively by DRIFT, 13C and 29Si solid state NMR, XPS, and photoluminescence. Zeta potential and contact angle measurements exhibited various surface properties (hydrophilic/hydrophobic balance and electric charge) according to the functional groups. Confocal laser scanning microscopy (CLSM) measurements showed that the spatial distribution of these nanoparticles inside a biofilm of Pseudomonas aeruginosa PAO1 depends more on their hydrophilic/hydrophobic characteristics than on their size. CLSM observations using two nanosized particles (25 and 68 nm) suggest that narrow diffusion paths exist through the extracellular polymeric substances matrix.

Item Type:Article
Additional Information:Thanks to Taylor & Francis editor. The definitive version is available at http://www.tandfonline.com The original PDF can be found at Biofouling website: http://www.tandfonline.com/doi/abs/10.1080/08927014.2013.798866#.Ul5E81BHAow
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP / São Paulo State University (BRAZIL)
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Deposited By: Vincent GERBAUD
Deposited On:16 Oct 2013 08:15

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