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Impact of oil on bacterial community structure in bioturbated sediments

Stauffert, Magali and Cravo-Laureau, Cristiana and Jezequel, Ronan and Barantal, Sandra and Cuny, Philippe and Gilbert, Franck and Cagnon, Christine and Militon, Cécile and Amouroux, David and Mahdaoui, Fatima and Bouyssiere, Brice and Stora, Georges and Merlin, François-Xavier and Duran, Robert Impact of oil on bacterial community structure in bioturbated sediments. (2013) PLoS ONE, 8 (6). 1-15. ISSN 1932-6203

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Official URL: http://dx.doi.org/10.1371/journal.pone.0065347

Abstract

Oil spills threaten coastlines where biological processes supply essential ecosystem services. Therefore, it is crucial to understand how oil influences the microbial communities in sediments that play key roles in ecosystem functioning. Ecosystems such as sediments are characterized by intensive bioturbation due to burrowing macrofauna that may modify the microbial metabolisms. It is thus essential to consider the bioturbation when determining the impact of oil on microbial communities. In this study, an experimental laboratory device maintaining pristine collected mudflat sediments in microcosms closer to true environmental conditions - with tidal cycles and natural seawater - was used to simulate an oil spill under bioturbation conditions. Different conditions were applied to the microcosms including an addition of: standardized oil (Blend Arabian Light crude oil, 25.6 mg.g21 wet sediment), the common burrowing organism Hediste (Nereis) diversicolor and both the oil and H. diversicolor. The addition of H. diversicolor and its associated bioturbation did not affect the removal of petroleum hydrocarbons. After 270 days, 60% of hydrocarbons had been removed in all microcosms irrespective of the H. diversicolor addition. However, 16S-rRNA gene and 16S-cDNA T-RFLP and RT-PCR-amplicon libraries analysis showed an effect of the condition on the bacterial community structure, composition, and dynamics, supported by PerMANOVA analysis. The 16S-cDNA libraries from microcosms where H. diversicolor was added (oiled and un-oiled) showed a marked dominance of sequences related to Gammaproteobacteria. However, in the oiled-library sequences associated to Deltaproteobacteria and Bacteroidetes were also highly represented. The 16S-cDNA libraries from oiled-microcosms (with and without H. diversicolor addition) revealed two distinct microbial communities characterized by different phylotypes associated to known hydrocarbonoclastic bacteria and dominated by Gammaproteobacteria and Deltaproteobacteria. In the oiled-microcosms, the addition of H. diversicolor reduced the phylotype-richness, sequences associated to Actinobacteria, Firmicutes and Plantomycetes were not detected. These observations highlight the influence of the bioturbation on the bacterial community structure without affecting the biodegradation capacities.

Item Type:Article
Additional Information:Traité par Liza
HAL Id:hal-00867612
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Other partners > Aix-Marseille Université - AMU (FRANCE)
Other partners > Centre de Documentation, de Recherche et d'Expérimentations sur les pollutions accidentelles des eaux - CEDRE (FRANCE)
French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
French research institutions > Institut de Recherche pour le Développement - IRD (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > Université du Sud Toulon-Var - USTV (FRANCE)
Other partners > Université de Pau et des Pays de l'Adour - UPPA (FRANCE)
Laboratory name:
Funders:
French ANR CESA Programme
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Deposited By: Franck Gilbert
Deposited On:30 Sep 2013 09:51

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