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Biocompatible polymer-assisted dispersion of multi walled carbon nanotubes in water, application to the investigation of their ecotoxicity using Xenopus laevis amphibian larvae

Bourdiol, Floriane and Mouchet, Florence and Perrault, Annie and Fourquaux, Isabelle and Datas, Lucien and Gancet, Christian and Boutonnet, Jean-Charles and Pinelli, Eric and Gauthier, Laury and Flahaut, Emmanuel Biocompatible polymer-assisted dispersion of multi walled carbon nanotubes in water, application to the investigation of their ecotoxicity using Xenopus laevis amphibian larvae. (2013) Carbon, 54. 175-191. ISSN 0008-6223

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Official URL: http://dx.doi.org/10.1016/j.carbon.2012.11.024

Abstract

Carbon nanotubes (CNTs) tend to readily agglomerate and settle down in water, while the adsorption of compounds present in natural aquatic media could enhance their dispersion and stabilization in the water column. We designed a new exposure protocol to compare the biological responses of Xenopus laevis larvae exposed in semi-static conditions to size-reduced agglomerates of multi-walled carbon nanotubes (MWCNTs) in suspension in the water column and/or to larger agglomerates. Suspensions were prepared using a combination of a non-covalent functionalization with a non-toxic polymer (either carboxymethylcellulose, CMC, or gum arabic, GA) and mechanical dispersion methods (mainly ultrasonication). The ingestion of agglomerates which have settled down was incriminated in the disruption of the intestinal transit and the assimilation of nutrients, leading to acute and chronic toxicities at the highest tested concentrations. Rise in mortality, decrease in the growth rate and induction of genotoxicity from low concentrations (1 mg/L in the presence of CMC) were evidenced in presence of suspended MWCNTs in the water column. The biological responses seemed to be modulated when GA, a potential antioxidant, was used. We hypothesized that MWCNTs should interfere mainly at the surface of the gills, acting as a potential respiratory toxicant and generally inducing indirect effects.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com The original PDF of the article can be found at http://dx.doi.org/10.1016/j.carbon.2012.11.024
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
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Deposited By: cirimat webmestre
Deposited On:15 Oct 2013 14:26

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