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Simultaneous assay of ascorbate and urate antioxidants in human blood serum using PEDOT-based electrocehmical microsensor

Sekli-Belaidi, Fadhila and Galinier, Anne and Gros, Pierre Simultaneous assay of ascorbate and urate antioxidants in human blood serum using PEDOT-based electrocehmical microsensor. (2013) Combinatorial Chemistry & High Throughput Screening, vol. 16 (n° 2). pp. 84-91. ISSN 1875-5402

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Official URL: http://dx.doi.org/10.2174/1386207311316020002

Abstract

An electrochemical microsensor has been developed for the simultaneous assay of ascorbate (AA) and urate (UA) antioxidants in human blood serum. The electrode surface was modified by means of electropolymerized conductive poly(3,4-ethylenedioxythiophene) PEDOT organic films. Highly selective responses (detection potential difference more than 250 mV) were obtained for both biomarkers by optimizing the PEDOT thickness. Contrary to most previous studies the analytical performances were recorded by testing the microsensor directly in blood serum without any pretreatment of the samples. Using differential pulse voltammetry (DPV) the sensitivity and detection limit were 0.481 µA µM-1 cm-2 and 4.2 µmol L-1 for AA and 1.815 µA µM-1 cm-2 and 2.0 µmol L-1 for UA. The calibration curves were linear in the concentration range 5–200 µmol L-1 for AA and 3–700 µmol L-1 for UA. The accuracy of the sensor was satisfactorily compared with the reference analytical methods provided that the synergic effect between both antioxidants was considered. The sensor response was unmodified in the presence of the major biochemical interfering species.

Item Type:Article
Additional Information:Thanks to Bentham Science Publishers. The original publication is available at http://www.benthamscience.com/cchts/
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > Centre Hospitalier Universitaire de Toulouse - CHU Toulouse (FRANCE)
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Deposited By: David Evrard
Deposited On:24 May 2013 13:29

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