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Design of double-walled carbon nanotubes for biomedical applications

Neves, Vera and Heister, Elena and Costa, Sara and Tîlmaciu, Carmen and Flahaut, Emmanuel and Soula, Brigitte and Coley, Helen M. and McFadden, Johnjoe and Silva, S. Ravi P. Design of double-walled carbon nanotubes for biomedical applications. (2012) Nanotechnology, 23 (36). 1-8. ISSN 0957-4484

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Official URL: http://dx.doi.org/10.1088/0957-4484/23/36/365102

Abstract

Double-walled carbon nanotubes (DWNTs) prepared by catalytic chemical vapour deposition were functionalized in such a way that they were optimally designed as a nano-vector for the delivery of small interfering RNA (siRNA), which is of great interest for biomedical research and drug development. DWNTs were initially oxidized and coated with a polypeptide (Poly(Lys:Phe)), which was then conjugated to thiol-modified siRNA using a heterobifunctional cross-linker. The obtained oxDWNT–siRNA was characterized by Raman spectroscopy inside and outside a biological environment (mammalian cells). Uptake of the custom designed nanotubes was not associated with detectable biochemical perturbations in cultured cells, but transfection of cells with DWNTs loaded with siRNA targeting the green fluorescent protein (GFP) gene, serving as a model system, as well as with therapeutic siRNA targeting the survivin gene, led to a significant gene silencing effect, and in the latter case a resulting apoptotic effect in cancer cells.

Item Type:Article
Additional Information:Thanks to Institute of Physics editor. The definitive version is available at http://iopscience.iop.org/0957-4484/23/36/365102/
HAL Id:hal-00858588
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Other partners > Szczecin University of Technology (POLAND)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > University of Surrey (UNITED KINGDOM)
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Deposited By: cirimat webmestre
Deposited On:05 Sep 2013 13:47

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