OATAO - Open Archive Toulouse Archive Ouverte Open Access Week

Self-assembling peptide-enriched electrospun polycaprolactone scaffolds promote the h-osteoblast adhesion and modulate differentiation-associated gene expression

Danesin, Roberta and Brun, Paola and Roso, Martina and Delaunay, Florian and Samouillan, Valérie and Brunelli, Katya and Iucci, Giovanna and Ghezzo, Francesca and Modesti, Michele and Castagliuolo, Ignazio and Dettin, Monica Self-assembling peptide-enriched electrospun polycaprolactone scaffolds promote the h-osteoblast adhesion and modulate differentiation-associated gene expression. (2012) Bone, 51 (5). 851-859. ISSN 8756-3282

[img]
Preview
(Document in English)

PDF (Author's version) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
490kB

Official URL: http://dx.doi.org/10.1016/j.bone.2012.08.119

Abstract

Electrospun polycaprolactone (PCL) is able to support the adhesion and growth of h-osteoblasts and to delay their degradation rate to a greater extent with respect to other polyesters. The drawbacks linked to its employment in regenerative medicine arise fromits hydrophobic nature and the lack of biochemical signals linked to it. This work reports on the attempt to add five different self-assembling (SA) peptides to PCL solutions before electrospinning. The hybrid scaffolds obtained had regular fibers (SEM analysis) whose diameters were similar to those of the extracellularmatrix, more stable hydrophilic (contact angle measurement) surfaces, and anamorphous phase constrained by peptides (DSC analysis). They appeared to have a notable capacity to promote the h-osteoblast adhesion and differentiation process by increasing the gene expression of alkaline phosphatase, bone sialoprotein, and osteopontin. Adding an Arg-Gly-Asp (RGD) motif to a self-assembling sequence was found to enhance cell adhesion, while the same motif condensed with a scrambled sequence did not, indicating that there is a cooperative effect between RGD and 3D architecture created by the self-assembling peptides. The study demonstrates that self-assembling peptide scaffolds are still able to promote beneficial effects on h-osteoblasts even after they have been included in electrospun polycaprolactone. The possibility of linking biochemical messages to self-assembling peptides could lead the way to a 3D decoration of fibrous scaffolds.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The original PDF of the article can be found at Bone website : http://www.sciencedirect.com/science/journal/87563282
HAL Id:hal-00904043
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > Università degli Studi Roma Tre (ITALY)
Other partners > Università degli Studi di Padova (ITALY)
Laboratory name:
Statistics:download
Deposited By: cirimat webmestre
Deposited On:09 Sep 2013 07:54

Repository Staff Only: item control page