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Evaluation of alginate microspheres for mesenchymal stem cell engraftment on solid organ

Trouche, Elodie and Girod Fullana, Sophie and Mias, Céline and Ceccaldi, Caroline and Tortosa, Florence and Seguelas, Marie Hélène and Calise, Denis and Parini, Angelo and Cussac, Daniel and Sallerin, Brigitte Evaluation of alginate microspheres for mesenchymal stem cell engraftment on solid organ. (2010) Cell Transplantation, 19 (12). 1623-1633. ISSN 0963-6897

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Official URL: http://dx.doi.org/10.3727/096368910X514297

Abstract

Mesenchymal stem cells (MSCs) may be used as a cell source for cell therapy of solid organs due to their differentiation potential and paracrine effect. Nevertheless, optimization of MSC-based therapy needs to develop alternative strategies to improve cell administration and efficiency. One option is the use of alginate microencapsulation, which presents an excellent biocompatibility and an in vivo stability. As MSCs are hypoimmunogenic, it was conceivable to produce microparticles with [alginate-poly-L-lysine-alginate (APA) microcapsules] or without (alginate microspheres) a surrounding protective membrane. Therefore, the aim of this study was to determine the most suitable microparticles to encapsulate MSCs for engraftment on solid organ. First, we compared the two types of microparticles with 4 × 106 MSCs/ml of alginate. Results showed that each microparticle has distinct morphology and mechanical resistance but both remained stable over time. However, as MSCs exhibited a better viability in microspheres than in microcapsules, the study was pursued with microspheres. We demonstrated that viable MSCs were still able to produce the paracrine factor bFGF and did not present any chondrogenic or osteogenic differentiation, processes sometimes reported with the use of polymers. We then proved that microspheres could be implanted under the renal capsule without degradation with time or inducing impairment of renal function. In conclusion, these microspheres behave as an implantable scaffold whose biological and functional properties could be adapted to fit with clinical applications.

Item Type:Article
Additional Information:Thanks to Cognizant Communication Corporation editor. The definitive version is available at Cell Transplantation website: http://www.ingentaconnect.com The original article can be found at http://www.ingentaconnect.com/content/cog/ct/2010/00000019/00000012/art00010
Audience (journal):International peer-reviewed journal
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Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
French research institutions > Institut National de la Santé et de la Recherche Médicale - INSERM (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
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Deposited By: cirimat webmestre
Deposited On:11 Sep 2013 10:59

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