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Human tropoelastin sequence: dynamics of polypeptide coded by exon 6 in solution

Tintar, Doris and Samouillan, Valérie and Dandurand, Jany and Lacabanne, Colette and Pepe, Antonietta and Bochicchio, Brigida and Tamburro, Antonio Mario Human tropoelastin sequence: dynamics of polypeptide coded by exon 6 in solution. (2009) Biopolymers, 91 (11). 943-952. ISSN 0006-3525

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Official URL: http://dx.doi.org/10.1002/bip.21282

Abstract

Calorimetric studies were performed on exon 6 in powdered form and in solution [water and 2,2,2- trifluoroethanol (TFE), a structure-inducing solvent or cosolvent]. Dynamic dielectric spectroscopy (DDS) analyses were realized in water and 20% TFE. The major role of solvent–peptide organization is evidenced with these techniques. Calorimetric measurements reveal the structural water organization around the polypeptide as well as the presence of hydrophobic interactions in TFE solution. Dielectric measurements showed for exon 6/ water a decrease of relaxations times of bulk solvent implying a faster dynamics with a slight increase of the activation entropy, suggesting that exon 6 probably creates disorder within the solvent. For TFE/water mixtures, an influence of exon 6 on its environment was seen with a relaxation associated with the exon 6/solvent interactions reinforced by storage of 72 h. Finally, exon 6/solvent interactions were clearly observed with addition of TFE.

Item Type:Article
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > Università degli Studi della Basilicata - UNIBAS (ITALY)
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Deposited By: cirimat webmestre
Deposited On:12 Sep 2013 06:33

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