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Human hepatic cell behavior on polysulfone membrane with double porosity level

Dufresne, Murielle and Bacchin, Patrice and Cerino, Giulia and Remigy, Jean-Christophe and Adrianus, Gerard Nathanael and Aimar, Pierre and Legallais, Cécile Human hepatic cell behavior on polysulfone membrane with double porosity level. (2013) Journal of Membrane Science, 428. 454-461. ISSN 0376-7388

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Official URL: http://dx.doi.org/10.1016/j.memsci.2012.10.041

Abstract

In the membrane-based bioartificial livers developed up to now,the hepatic cells were located either in the fibers lumen or in the cartridge,with limited capacity for cell hosting.Here,we designed a polysulfone (PSU) membrane with a double porosity level:(i)surface macroporosity emerging in macrochambers accessible to hepatic cell colonization;(ii)microporosity to ensure gas and molecule transfers between macrochambers and supernatant,as well as potential immune barrier. ESEM and X-ray tomography confirmed that macrochambers accessed the membrane surface and were inter-connected. Biocompatibility and performances of this PSU membrane with double porosity level were compared to classical semi-permeable structures,following cell organization,cell proliferation and liver specific activities over a 9 days incubation. Macrochambers were colonized by hepatic cells, leading to higher albumin synthesis compared to control.Therefore,this membrane with double porosity appeared as a promising support to offer an inner 3D environment adequate to cell proliferation to form a liver-like tissue.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com The original PDF of the article can be found at Journal of Membrane Science website : http://dx.doi.org/10.1016/j.memsci.2012.10.041
HAL Id:hal-00788387
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > Université de Technologie de Compiègne – UTC (FRANCE)
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Deposited By: Patrice Bacchin
Deposited On:13 Feb 2013 13:26

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