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Human hepatic cell behavior on polysulfone membrane with double porosity level

Dufresne, Murielle and Bacchin, Patrice and Cerino, Giulia and Remigy, Jean-Christophe and Adrianus, Gerard Nathanael and Aimar, Pierre and Legallais, Cécile Human hepatic cell behavior on polysulfone membrane with double porosity level. (2013) Journal of Membrane Science, vol. 428. pp. 454-461. ISSN 0376-7388

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Official URL: http://dx.doi.org/10.1016/j.memsci.2012.10.041

Abstract

In the membrane-based bioartificial livers developed up to now,the hepatic cells were located either in the fibers lumen or in the cartridge,with limited capacity for cell hosting.Here,we designed a polysulfone (PSU) membrane with a double porosity level:(i)surface macroporosity emerging in macrochambers accessible to hepatic cell colonization;(ii)microporosity to ensure gas and molecule transfers between macrochambers and supernatant,as well as potential immune barrier. ESEM and X-ray tomography confirmed that macrochambers accessed the membrane surface and were inter-connected. Biocompatibility and performances of this PSU membrane with double porosity level were compared to classical semi-permeable structures,following cell organization,cell proliferation and liver specific activities over a 9 days incubation. Macrochambers were colonized by hepatic cells, leading to higher albumin synthesis compared to control.Therefore,this membrane with double porosity appeared as a promising support to offer an inner 3D environment adequate to cell proliferation to form a liver-like tissue.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com The original PDF of the article can be found at Journal of Membrane Science website : http://dx.doi.org/10.1016/j.memsci.2012.10.041
HAL Id:hal-00788387
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS (FRANCE)
Other partners > Université de Technologie de Compiègne – UTC (FRANCE)
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Deposited By: Patrice Bacchin
Deposited On:13 Feb 2013 13:26

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