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Non-conventional gas phase remediation of volatile halogenated compounds by dehydrated bacteria

Erable, Benjamin and Goubet, Isabelle and Seltana, Amira and Maugard, Thierry Non-conventional gas phase remediation of volatile halogenated compounds by dehydrated bacteria. (2009) Journal of environmental management, vol. 90 (n° 8). pp. 2841-2844. ISSN 1095-8630

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Official URL: http://dx.doi.org/10.1016/j.jenvman.2009.01.010

Abstract

Traditional biological removal processes are limited by the low solubility of halogenated compounds in aqueous media. A new technology appears very suitable for the remediation of these volatile organic compounds (VOCs). Solid/gas bio-catalysis applied in VOC remediation can transform halogenated compounds directly in the gas phase using dehydrated cells as a bio-catalyst. The hydrolysis of volatile halogenated substrates into the corresponding alcohol was studied in a solid/gas biofilter where lyophilised bacterial cultures were used as the catalyst. Four strains containing dehalogenase enzymes were tested for the hydrolysis of 1-chlorobutane. The highest removal yield was obtained using the dhaA-containing strains, the maximal reaction rate of 0.8 micromol min(-1)g(-1) being observed with Escherichia coli BL21(DE3)(dhaA). Various treatments such as cell disruption by lysozyme or alkaline gas addition in the bio-filter could stabilise the dehalogenase activity of the bacteria. A pre-treatment of the dehydrated bacterial cells by ammonia vapour improved the stability of the catalyst and a removal activity of 0.9 micromol min(-1)g(-1) was then obtained for 60h. Finally, the process was extended to a range of halogenated substrates including bromo- and chloro-substrates. It was shown that the removal capacity for long halogenated compounds (C(5)-C(6)) was greatly increased relative to traditional biological processes.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com The original PDF of the article can be found at Journal of environmental management website : http://dx.doi.org/10.1016/j.jenvman.2009.01.010
Audience (journal):International peer-reviewed journal
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Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS
Other partners > Université de La Rochelle (FRANCE)
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Deposited By:Benjamin ERABLE

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