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Adhesive factor/rabbit 2, a new fimbrial adhesin and a virulence factor from Escherichia coli O103, a serogroup enteropathogenic for rabbits

Fiederling, Franck and Boury, Michèle and Petit, Claude and Milon, Alain Adhesive factor/rabbit 2, a new fimbrial adhesin and a virulence factor from Escherichia coli O103, a serogroup enteropathogenic for rabbits. (1997) Infection and Immunity, vol. 6 (n° 2). pp. 847-851. ISSN 0019-9567

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Official URL: http://iai.asm.org/cgi/reprint/65/2/847

Abstract

Enteropathogenic Escherichia coli-like E. coli strains belonging to serovar O103:K-:H2 and rhamnose-negative biotypes are highly pathogenic diarrhea-inducing strains for weaned European rabbits. We describe here the cloning and sequencing of the major subunit gene of a new fimbrial adhesin, adhesive factor/rabbit 2 (AF/R2), which confers on these strains the ability to attach to rabbit enterocytes and to HeLa cells in a diffuse manner and which is associated with in vivo virulence. The chromosomal operon that encodes functional AF/R2 has been cloned from strain B10. The major subunit gene afr2G, as well as an adjacent open reading frame, afr2H, has been sequenced. The Afr2G protein shows homologies with FaeG and ClpG, which are the respective major subunits of fimbrial adhesin K88 (F4) and afimbrial adhesin CS31A. Plasmid carrying the operon transcomplements an AF/R2-negative TnphoA mutant for its ability to express AF/R2. As a whole, AF/R2 is a new member of the E. coli K88 adhesin family which is associated with virulence and which may serve in the design of vaccines.

Item Type:Article
Additional Information:Thanks to American Society for Microbiology. The original PDF of the article can be found at Infection and Immunity website : http://iai.asm.org
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Institut National de la Recherche Agronomique - INRA
Université de Toulouse > Ecole Nationale Vétérinaire de Toulouse - ENVT
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Deposited By: Mathieu Andro
Deposited On:19 Jun 2008 08:28

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