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Intimin, tir, and shiga toxin 1 do not influence enteropathogenic responses to shiga toxin-producing Escherichia coli in bovine ligated intestinal loops

Stevens, Mark P. and Marchès, Olivier and Campbell, June and Huter, Veronika and Frankel, Gad and Phillips, Alan D. and Oswald, Eric and Wallis, Timothy S. Intimin, tir, and shiga toxin 1 do not influence enteropathogenic responses to shiga toxin-producing Escherichia coli in bovine ligated intestinal loops. (2002) Infection and immunity, vol. 7 (n° 2). pp. 945-52. ISSN 0019-9567

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Official URL: http://iai.asm.org/cgi/reprint/70/2/945

Abstract

Shiga toxin-producing Escherchia coli (STEC) comprises a group of attaching and effacing (A/E) enteric pathogens of animals and humans. Natural and experimental infection of calves with STEC may result in acute enteritis or subclinical infection, depending on serotype- and host-specific factors. To quantify intestinal secretory and inflammatory responses to STEC in the bovine intestine, serotypes that are associated with human disease (O103:H2 and O157:H7) were introduced into ligated mid-ileal loops in gnotobiotic and conventional calves, and fluid accumulation and recruitment of radiolabeled neutrophils were measured after 12 h. STEC serotype O103:H2, but not serotype O157:H7, elicited strong enteropathogenic responses. To determine if the inflammatory response to STEC O103:H2 in calves requires Shiga toxin 1 or intimate bacterial attachment to the intestinal epithelium, defined mutations were made in the stx1, eae, and tir genes. Our data indicate that some STEC induce intestinal inflammatory responses in calves by a mechanism that is independent of A/E-lesion formation, intimin, or Shiga toxin 1. This may have implications for strategies to reduce STEC carriage in cattle.

Item Type:Article
Additional Information:Thanks to American Society for Microbiology. The original PDF of the article can be found at Infection and immunity website : http://iai.asm.org
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Other partners > Imperial College of Science, Technology and Medicine (UNITED KINGDOM)
French research institutions > Institut National de la Recherche Agronomique - INRA
Other partners > Royal Free Hospital (UNITED KINGDOM)
Other partners > Institute for Animal Health (UNITED KINGDOM)
Université de Toulouse > Ecole Nationale Vétérinaire de Toulouse - ENVT
Laboratory name:
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Deposited By: Mathieu Andro

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