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Imaging of DNA hybridization on microscopic polypyrrole patterns using scanning electrochemical microscopy (SECM): the HRP bio-catalyzed oxidation of 4-chloro-1-naphthol

Fortin, Elodie and Mailley, Pascale and Lacroix, Loïc and Szunerits, Sabine Imaging of DNA hybridization on microscopic polypyrrole patterns using scanning electrochemical microscopy (SECM): the HRP bio-catalyzed oxidation of 4-chloro-1-naphthol. (2006) The Analyst, 131 (2). 186-193. ISSN 0003-2654

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Official URL: http://dx.doi.org/10.1039/b504711k

Abstract

We illustrate in this paper the successful combination of the direct and feedback mode of scanning electrochemical microscopy (SECM) for the writing of oligonucleotide patterns on thin gold films alongside the imaging of DNA hybridization. The patterning process was achieved using the direct mode of SECM, where the electrical field established between the SECM tip and the gold interface was used to drive the local deposition of micrometre sized polypyrrole spots to which a 15mer oligonucleotide (ODN) strand was linked covalently. Imaging of the deposited polypyrrole-ODNs was achieved by means of the feedback mode of SECM using Ru(NH3)63+ as the mediator. The detection of the hybridization reaction of the ODN probes with their biotinylated complementary strands using SECM was possible after subsequent reactions with streptavidin and biotinylated horseradish peroxidase (HRP). The HRP-biocatalyzed oxidation of 4-chloro-1-naphthol (1) in the presence of H2O2, and the precipitation of the insoluble product 4-chloro-1-naphthon (2) on the hybridized areas on the gold film caused a local alteration of conductivity. Such a change in conductivity was sensitively detected by the SECM tip and allowed imaging of DNA arrays in a fast and straightforward way.

Item Type:Article
Additional Information:Thanks to Royal society of chemistry editor. The definitive version is available at : http://dx.doi.org/10.1039/b504711k
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Commissariat à l'Energie Atomique et aux énergies alternatives - CEA (FRANCE)
French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Other partners > Institut polytechnique de Grenoble (FRANCE)
Other partners > Université Joseph Fourier Grenoble 1 - UJF (FRANCE)
Other partners > Université de Savoie Mont Blanc - USMB (FRANCE)
Laboratory name:
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Deposited By: Loic LACROIX
Deposited On:14 Jan 2014 14:24

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