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Application of a reversed-phase HPLC method for quantitative P-Coumaric acid analysis in wine

Salameh, Dominique and Brandam, Cédric and Rizk, Toufic and Lteif, Roger and Strehaiano, Pierre Application of a reversed-phase HPLC method for quantitative P-Coumaric acid analysis in wine. (2010) Journal international des sciences de la vigne et du vin, vol. 44 (n° 2). pp. 117-125. ISSN 1151-0285

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Abstract

Aims: This paper presents a rapid chromatographic method to monitor the concentration of p-coumaric acid in wine and in bioconversion studies. Methods and results: RP-HPLC method was validated in synthetic wine medium and in natural red wine. Mobile phase composition was water 77%, acetonitrile 23%. Formic acid was added to control pH at 3.5. The flow was 0.7 mL/min and the temperature 30 °C. The detection was done using UV at 305 nm. The linearity range was validated between 0.5 and 15 mg/L. The resolution was respectively 5.35 and 2.99. The detection and quantification limits were 0.01 mg/L and 0.04 mg/L. This method was used to study p-coumaric acid bioconversion into 4-ethylphenol and 4-vinylphenol, and to study this acid adsorption in enological conditions. Conclusions: This paper presented a simple HPLC method to monitor the concentration of p-coumaric acid in synthetic media and natural wine. It was used to study the p-coumaric acid bioconversion rates and mechanism. Significance and impact of the study: This method is useful to monitor p-coumaric acid concentration, which helps to predict amounts of 4-ethylphenol or 4-vinylphenol that can be produced in wine. This method can be helpful to control undesirable phenolic flavors potential in wine.

Item Type:Article
Additional Information:Thanks to Vigne et Vin Publications Internationales editor. The definitive version is available at http://www.jisvv.com The original PDF of the article can be found at : http://www.jisvv.com/moteur/moteur_avance.php#
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS (FRANCE)
Other partners > Université Saint-Joseph (LEBANON)
Laboratory name:
Laboratoire de Génie Chimique - LGC (Toulouse, France) - Bioprocédés et systèmes microbiens (BioSyM)
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Deposited By: Audrey LEFEVRE
Deposited On:04 Mar 2013 16:01

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